Novel small RNA spike-in oligonucleotides enable absolute normalization of small RNA-Seq data

Abstract Normalization of high-throughput small RNA sequencing (sRNA-Seq) data is required to compare sRNA levels across different samples. Commonly used relative normalization approaches can cause erroneous conclusions due to fluctuating small RNA populations between tissues. We developed a set of...

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Autores principales:	Stefan Lutzmayer, Balaji Enugutti, Michael D. Nodine
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/17fc805a814547d18b623e1269983cae
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spelling	oai:doaj.org-article:17fc805a814547d18b623e1269983cae2021-12-02T16:08:11ZNovel small RNA spike-in oligonucleotides enable absolute normalization of small RNA-Seq data10.1038/s41598-017-06174-32045-2322https://doaj.org/article/17fc805a814547d18b623e1269983cae2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06174-3https://doaj.org/toc/2045-2322Abstract Normalization of high-throughput small RNA sequencing (sRNA-Seq) data is required to compare sRNA levels across different samples. Commonly used relative normalization approaches can cause erroneous conclusions due to fluctuating small RNA populations between tissues. We developed a set of sRNA spike-in oligonucleotides (sRNA spike-ins) that enable absolute normalization of sRNA-Seq data across independent experiments, as well as the genome-wide estimation of sRNA:mRNA stoichiometries when used together with mRNA spike-in oligonucleotides.Stefan LutzmayerBalaji EnuguttiMichael D. NodineNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-6 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stefan Lutzmayer Balaji Enugutti Michael D. Nodine Novel small RNA spike-in oligonucleotides enable absolute normalization of small RNA-Seq data
description	Abstract Normalization of high-throughput small RNA sequencing (sRNA-Seq) data is required to compare sRNA levels across different samples. Commonly used relative normalization approaches can cause erroneous conclusions due to fluctuating small RNA populations between tissues. We developed a set of sRNA spike-in oligonucleotides (sRNA spike-ins) that enable absolute normalization of sRNA-Seq data across independent experiments, as well as the genome-wide estimation of sRNA:mRNA stoichiometries when used together with mRNA spike-in oligonucleotides.
format	article
author	Stefan Lutzmayer Balaji Enugutti Michael D. Nodine
author_facet	Stefan Lutzmayer Balaji Enugutti Michael D. Nodine
author_sort	Stefan Lutzmayer
title	Novel small RNA spike-in oligonucleotides enable absolute normalization of small RNA-Seq data
title_short	Novel small RNA spike-in oligonucleotides enable absolute normalization of small RNA-Seq data
title_full	Novel small RNA spike-in oligonucleotides enable absolute normalization of small RNA-Seq data
title_fullStr	Novel small RNA spike-in oligonucleotides enable absolute normalization of small RNA-Seq data
title_full_unstemmed	Novel small RNA spike-in oligonucleotides enable absolute normalization of small RNA-Seq data
title_sort	novel small rna spike-in oligonucleotides enable absolute normalization of small rna-seq data
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/17fc805a814547d18b623e1269983cae
work_keys_str_mv	AT stefanlutzmayer novelsmallrnaspikeinoligonucleotidesenableabsolutenormalizationofsmallrnaseqdata AT balajienugutti novelsmallrnaspikeinoligonucleotidesenableabsolutenormalizationofsmallrnaseqdata AT michaeldnodine novelsmallrnaspikeinoligonucleotidesenableabsolutenormalizationofsmallrnaseqdata
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Novel small RNA spike-in oligonucleotides enable absolute normalization of small RNA-Seq data

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