Characterization of eukaryotic DNA N6-methyladenine by a highly sensitive restriction enzyme-assisted sequencing

DNA N6-methyladenine is prevalent in prokaryotes, and is recently also detected in eukaryotes such as roundworm and fly. Here, Luo et al. report a DpnI-assisted base resolution method that detects 6mA genome-wide with nanograms of input DNA and lower sequencing depth than the previous restriction en...

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Autores principales:	Guan-Zheng Luo, Fang Wang, Xiaocheng Weng, Kai Chen, Ziyang Hao, Miao Yu, Xin Deng, Jianzhao Liu, Chuan He
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/18aa662e4dcb4b83b7acf84a73004a33
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spelling	oai:doaj.org-article:18aa662e4dcb4b83b7acf84a73004a332021-12-02T14:40:14ZCharacterization of eukaryotic DNA N6-methyladenine by a highly sensitive restriction enzyme-assisted sequencing10.1038/ncomms113012041-1723https://doaj.org/article/18aa662e4dcb4b83b7acf84a73004a332016-04-01T00:00:00Zhttps://doi.org/10.1038/ncomms11301https://doaj.org/toc/2041-1723DNA N6-methyladenine is prevalent in prokaryotes, and is recently also detected in eukaryotes such as roundworm and fly. Here, Luo et al. report a DpnI-assisted base resolution method that detects 6mA genome-wide with nanograms of input DNA and lower sequencing depth than the previous restriction enzyme-based approach.Guan-Zheng LuoFang WangXiaocheng WengKai ChenZiyang HaoMiao YuXin DengJianzhao LiuChuan HeNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-6 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Guan-Zheng Luo Fang Wang Xiaocheng Weng Kai Chen Ziyang Hao Miao Yu Xin Deng Jianzhao Liu Chuan He Characterization of eukaryotic DNA N6-methyladenine by a highly sensitive restriction enzyme-assisted sequencing
description	DNA N6-methyladenine is prevalent in prokaryotes, and is recently also detected in eukaryotes such as roundworm and fly. Here, Luo et al. report a DpnI-assisted base resolution method that detects 6mA genome-wide with nanograms of input DNA and lower sequencing depth than the previous restriction enzyme-based approach.
format	article
author	Guan-Zheng Luo Fang Wang Xiaocheng Weng Kai Chen Ziyang Hao Miao Yu Xin Deng Jianzhao Liu Chuan He
author_facet	Guan-Zheng Luo Fang Wang Xiaocheng Weng Kai Chen Ziyang Hao Miao Yu Xin Deng Jianzhao Liu Chuan He
author_sort	Guan-Zheng Luo
title	Characterization of eukaryotic DNA N6-methyladenine by a highly sensitive restriction enzyme-assisted sequencing
title_short	Characterization of eukaryotic DNA N6-methyladenine by a highly sensitive restriction enzyme-assisted sequencing
title_full	Characterization of eukaryotic DNA N6-methyladenine by a highly sensitive restriction enzyme-assisted sequencing
title_fullStr	Characterization of eukaryotic DNA N6-methyladenine by a highly sensitive restriction enzyme-assisted sequencing
title_full_unstemmed	Characterization of eukaryotic DNA N6-methyladenine by a highly sensitive restriction enzyme-assisted sequencing
title_sort	characterization of eukaryotic dna n6-methyladenine by a highly sensitive restriction enzyme-assisted sequencing
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/18aa662e4dcb4b83b7acf84a73004a33
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Characterization of eukaryotic DNA N6-methyladenine by a highly sensitive restriction enzyme-assisted sequencing

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