Premature differentiation of nephron progenitor cell and dysregulation of gene pathways critical to kidney development in a model of preterm birth

Abstract Preterm birth is a leading cause of neonatal morbidity. Survivors have a greater risk for kidney dysfunction and hypertension. Little is known about the molecular changes that occur in the kidney of individuals born preterm. Here, we demonstrate that mice delivered two days prior to full te...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Aleksandra Cwiek, Masako Suzuki, Kimberly deRonde, Mark Conaway, Kevin M. Bennett, Samir El Dahr, Kimberly J. Reidy, Jennifer R. Charlton
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/18b43f87c303431d9d6ae315e4174c3d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:18b43f87c303431d9d6ae315e4174c3d
record_format	dspace
spelling	oai:doaj.org-article:18b43f87c303431d9d6ae315e4174c3d2021-11-08T10:53:26ZPremature differentiation of nephron progenitor cell and dysregulation of gene pathways critical to kidney development in a model of preterm birth10.1038/s41598-021-00489-y2045-2322https://doaj.org/article/18b43f87c303431d9d6ae315e4174c3d2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-00489-yhttps://doaj.org/toc/2045-2322Abstract Preterm birth is a leading cause of neonatal morbidity. Survivors have a greater risk for kidney dysfunction and hypertension. Little is known about the molecular changes that occur in the kidney of individuals born preterm. Here, we demonstrate that mice delivered two days prior to full term gestation undergo premature cessation of nephrogenesis, resulting in a lower glomerular density. Kidneys from preterm and term groups exhibited differences in gene expression profiles at 20- and 27-days post-conception, including significant differences in the expression of fat-soluble vitamin-related genes. Kidneys of the preterm mice exhibited decreased proportions of endothelial cells and a lower expression of genes promoting angiogenesis compared to the term group. Kidneys from the preterm mice also had altered nephron progenitor subpopulations, early Six2 depletion, and altered Jag1 expression in the nephrogenic zone, consistent with premature differentiation of nephron progenitor cells. In conclusion, preterm birth alone was sufficient to shorten the duration of nephrogenesis and cause premature differentiation of nephron progenitor cells. These candidate genes and pathways may provide targets to improve kidney health in preterm infants.Aleksandra CwiekMasako SuzukiKimberly deRondeMark ConawayKevin M. BennettSamir El DahrKimberly J. ReidyJennifer R. CharltonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aleksandra Cwiek Masako Suzuki Kimberly deRonde Mark Conaway Kevin M. Bennett Samir El Dahr Kimberly J. Reidy Jennifer R. Charlton Premature differentiation of nephron progenitor cell and dysregulation of gene pathways critical to kidney development in a model of preterm birth
description	Abstract Preterm birth is a leading cause of neonatal morbidity. Survivors have a greater risk for kidney dysfunction and hypertension. Little is known about the molecular changes that occur in the kidney of individuals born preterm. Here, we demonstrate that mice delivered two days prior to full term gestation undergo premature cessation of nephrogenesis, resulting in a lower glomerular density. Kidneys from preterm and term groups exhibited differences in gene expression profiles at 20- and 27-days post-conception, including significant differences in the expression of fat-soluble vitamin-related genes. Kidneys of the preterm mice exhibited decreased proportions of endothelial cells and a lower expression of genes promoting angiogenesis compared to the term group. Kidneys from the preterm mice also had altered nephron progenitor subpopulations, early Six2 depletion, and altered Jag1 expression in the nephrogenic zone, consistent with premature differentiation of nephron progenitor cells. In conclusion, preterm birth alone was sufficient to shorten the duration of nephrogenesis and cause premature differentiation of nephron progenitor cells. These candidate genes and pathways may provide targets to improve kidney health in preterm infants.
format	article
author	Aleksandra Cwiek Masako Suzuki Kimberly deRonde Mark Conaway Kevin M. Bennett Samir El Dahr Kimberly J. Reidy Jennifer R. Charlton
author_facet	Aleksandra Cwiek Masako Suzuki Kimberly deRonde Mark Conaway Kevin M. Bennett Samir El Dahr Kimberly J. Reidy Jennifer R. Charlton
author_sort	Aleksandra Cwiek
title	Premature differentiation of nephron progenitor cell and dysregulation of gene pathways critical to kidney development in a model of preterm birth
title_short	Premature differentiation of nephron progenitor cell and dysregulation of gene pathways critical to kidney development in a model of preterm birth
title_full	Premature differentiation of nephron progenitor cell and dysregulation of gene pathways critical to kidney development in a model of preterm birth
title_fullStr	Premature differentiation of nephron progenitor cell and dysregulation of gene pathways critical to kidney development in a model of preterm birth
title_full_unstemmed	Premature differentiation of nephron progenitor cell and dysregulation of gene pathways critical to kidney development in a model of preterm birth
title_sort	premature differentiation of nephron progenitor cell and dysregulation of gene pathways critical to kidney development in a model of preterm birth
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/18b43f87c303431d9d6ae315e4174c3d
work_keys_str_mv	AT aleksandracwiek prematuredifferentiationofnephronprogenitorcellanddysregulationofgenepathwayscriticaltokidneydevelopmentinamodelofpretermbirth AT masakosuzuki prematuredifferentiationofnephronprogenitorcellanddysregulationofgenepathwayscriticaltokidneydevelopmentinamodelofpretermbirth AT kimberlyderonde prematuredifferentiationofnephronprogenitorcellanddysregulationofgenepathwayscriticaltokidneydevelopmentinamodelofpretermbirth AT markconaway prematuredifferentiationofnephronprogenitorcellanddysregulationofgenepathwayscriticaltokidneydevelopmentinamodelofpretermbirth AT kevinmbennett prematuredifferentiationofnephronprogenitorcellanddysregulationofgenepathwayscriticaltokidneydevelopmentinamodelofpretermbirth AT samireldahr prematuredifferentiationofnephronprogenitorcellanddysregulationofgenepathwayscriticaltokidneydevelopmentinamodelofpretermbirth AT kimberlyjreidy prematuredifferentiationofnephronprogenitorcellanddysregulationofgenepathwayscriticaltokidneydevelopmentinamodelofpretermbirth AT jenniferrcharlton prematuredifferentiationofnephronprogenitorcellanddysregulationofgenepathwayscriticaltokidneydevelopmentinamodelofpretermbirth
_version_	1718442500157341696

Premature differentiation of nephron progenitor cell and dysregulation of gene pathways critical to kidney development in a model of preterm birth

Ejemplares similares