Ultra-sensitive digital quantification of proteins and mRNA in single cells

Digital proximity ligation assay (dPLA) can measure proteins and mRNAs in single cells, but is not compatible with cell imaging and cannot quantify rare proteins due to a high dilution factor. Here the authors present an automated microfluidic device that combines live-cell imaging, chemical stimula...

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Autores principales:	Jing Lin, Christian Jordi, Minjun Son, Hoang Van Phan, Nir Drayman, Mustafa Fatih Abasiyanik, Luke Vistain, Hsiung-Lin Tu, Savaş Tay
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/1a00989204844592904106524e044438
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spelling	oai:doaj.org-article:1a00989204844592904106524e0444382021-12-02T14:39:35ZUltra-sensitive digital quantification of proteins and mRNA in single cells10.1038/s41467-019-11531-z2041-1723https://doaj.org/article/1a00989204844592904106524e0444382019-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-11531-zhttps://doaj.org/toc/2041-1723Digital proximity ligation assay (dPLA) can measure proteins and mRNAs in single cells, but is not compatible with cell imaging and cannot quantify rare proteins due to a high dilution factor. Here the authors present an automated microfluidic device that combines live-cell imaging, chemical stimulation, and dPLA in a smaller reaction volume.Jing LinChristian JordiMinjun SonHoang Van PhanNir DraymanMustafa Fatih AbasiyanikLuke VistainHsiung-Lin TuSavaş TayNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jing Lin Christian Jordi Minjun Son Hoang Van Phan Nir Drayman Mustafa Fatih Abasiyanik Luke Vistain Hsiung-Lin Tu Savaş Tay Ultra-sensitive digital quantification of proteins and mRNA in single cells
description	Digital proximity ligation assay (dPLA) can measure proteins and mRNAs in single cells, but is not compatible with cell imaging and cannot quantify rare proteins due to a high dilution factor. Here the authors present an automated microfluidic device that combines live-cell imaging, chemical stimulation, and dPLA in a smaller reaction volume.
format	article
author	Jing Lin Christian Jordi Minjun Son Hoang Van Phan Nir Drayman Mustafa Fatih Abasiyanik Luke Vistain Hsiung-Lin Tu Savaş Tay
author_facet	Jing Lin Christian Jordi Minjun Son Hoang Van Phan Nir Drayman Mustafa Fatih Abasiyanik Luke Vistain Hsiung-Lin Tu Savaş Tay
author_sort	Jing Lin
title	Ultra-sensitive digital quantification of proteins and mRNA in single cells
title_short	Ultra-sensitive digital quantification of proteins and mRNA in single cells
title_full	Ultra-sensitive digital quantification of proteins and mRNA in single cells
title_fullStr	Ultra-sensitive digital quantification of proteins and mRNA in single cells
title_full_unstemmed	Ultra-sensitive digital quantification of proteins and mRNA in single cells
title_sort	ultra-sensitive digital quantification of proteins and mrna in single cells
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/1a00989204844592904106524e044438
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Ultra-sensitive digital quantification of proteins and mRNA in single cells

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