Ultra-sensitive digital quantification of proteins and mRNA in single cells

Digital proximity ligation assay (dPLA) can measure proteins and mRNAs in single cells, but is not compatible with cell imaging and cannot quantify rare proteins due to a high dilution factor. Here the authors present an automated microfluidic device that combines live-cell imaging, chemical stimula...

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Autores principales: Jing Lin, Christian Jordi, Minjun Son, Hoang Van Phan, Nir Drayman, Mustafa Fatih Abasiyanik, Luke Vistain, Hsiung-Lin Tu, Savaş Tay
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/1a00989204844592904106524e044438
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