Transmission of equine influenza virus during an outbreak is characterized by frequent mixed infections and loose transmission bottlenecks.

The ability of influenza A viruses (IAVs) to cross species barriers and evade host immunity is a major public health concern. Studies on the phylodynamics of IAVs across different scales - from the individual to the population - are essential for devising effective measures to predict, prevent or co...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Joseph Hughes, Richard C Allen, Marc Baguelin, Katie Hampson, Gregory J Baillie, Debra Elton, J Richard Newton, Paul Kellam, James L N Wood, Edward C Holmes, Pablo R Murcia
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1a595681962f44f1a34f5aa8911af3ed
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1a595681962f44f1a34f5aa8911af3ed
record_format	dspace
spelling	oai:doaj.org-article:1a595681962f44f1a34f5aa8911af3ed2021-11-18T06:06:14ZTransmission of equine influenza virus during an outbreak is characterized by frequent mixed infections and loose transmission bottlenecks.1553-73661553-737410.1371/journal.ppat.1003081https://doaj.org/article/1a595681962f44f1a34f5aa8911af3ed2012-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23308065/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374The ability of influenza A viruses (IAVs) to cross species barriers and evade host immunity is a major public health concern. Studies on the phylodynamics of IAVs across different scales - from the individual to the population - are essential for devising effective measures to predict, prevent or contain influenza emergence. Understanding how IAVs spread and evolve during outbreaks is critical for the management of epidemics. Reconstructing the transmission network during a single outbreak by sampling viral genetic data in time and space can generate insights about these processes. Here, we obtained intra-host viral sequence data from horses infected with equine influenza virus (EIV) to reconstruct the spread of EIV during a large outbreak. To this end, we analyzed within-host viral populations from sequences covering 90% of the infected yards. By combining gene sequence analyses with epidemiological data, we inferred a plausible transmission network, in turn enabling the comparison of transmission patterns during the course of the outbreak and revealing important epidemiological features that were not apparent using either approach alone. The EIV populations displayed high levels of genetic diversity, and in many cases we observed distinct viral populations containing a dominant variant and a number of related minor variants that were transmitted between infectious horses. In addition, we found evidence of frequent mixed infections and loose transmission bottlenecks in these naturally occurring populations. These frequent mixed infections likely influence the size of epidemics.Joseph HughesRichard C AllenMarc BaguelinKatie HampsonGregory J BaillieDebra EltonJ Richard NewtonPaul KellamJames L N WoodEdward C HolmesPablo R MurciaPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 8, Iss 12, p e1003081 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Joseph Hughes Richard C Allen Marc Baguelin Katie Hampson Gregory J Baillie Debra Elton J Richard Newton Paul Kellam James L N Wood Edward C Holmes Pablo R Murcia Transmission of equine influenza virus during an outbreak is characterized by frequent mixed infections and loose transmission bottlenecks.
description	The ability of influenza A viruses (IAVs) to cross species barriers and evade host immunity is a major public health concern. Studies on the phylodynamics of IAVs across different scales - from the individual to the population - are essential for devising effective measures to predict, prevent or contain influenza emergence. Understanding how IAVs spread and evolve during outbreaks is critical for the management of epidemics. Reconstructing the transmission network during a single outbreak by sampling viral genetic data in time and space can generate insights about these processes. Here, we obtained intra-host viral sequence data from horses infected with equine influenza virus (EIV) to reconstruct the spread of EIV during a large outbreak. To this end, we analyzed within-host viral populations from sequences covering 90% of the infected yards. By combining gene sequence analyses with epidemiological data, we inferred a plausible transmission network, in turn enabling the comparison of transmission patterns during the course of the outbreak and revealing important epidemiological features that were not apparent using either approach alone. The EIV populations displayed high levels of genetic diversity, and in many cases we observed distinct viral populations containing a dominant variant and a number of related minor variants that were transmitted between infectious horses. In addition, we found evidence of frequent mixed infections and loose transmission bottlenecks in these naturally occurring populations. These frequent mixed infections likely influence the size of epidemics.
format	article
author	Joseph Hughes Richard C Allen Marc Baguelin Katie Hampson Gregory J Baillie Debra Elton J Richard Newton Paul Kellam James L N Wood Edward C Holmes Pablo R Murcia
author_facet	Joseph Hughes Richard C Allen Marc Baguelin Katie Hampson Gregory J Baillie Debra Elton J Richard Newton Paul Kellam James L N Wood Edward C Holmes Pablo R Murcia
author_sort	Joseph Hughes
title	Transmission of equine influenza virus during an outbreak is characterized by frequent mixed infections and loose transmission bottlenecks.
title_short	Transmission of equine influenza virus during an outbreak is characterized by frequent mixed infections and loose transmission bottlenecks.
title_full	Transmission of equine influenza virus during an outbreak is characterized by frequent mixed infections and loose transmission bottlenecks.
title_fullStr	Transmission of equine influenza virus during an outbreak is characterized by frequent mixed infections and loose transmission bottlenecks.
title_full_unstemmed	Transmission of equine influenza virus during an outbreak is characterized by frequent mixed infections and loose transmission bottlenecks.
title_sort	transmission of equine influenza virus during an outbreak is characterized by frequent mixed infections and loose transmission bottlenecks.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/1a595681962f44f1a34f5aa8911af3ed
work_keys_str_mv	AT josephhughes transmissionofequineinfluenzavirusduringanoutbreakischaracterizedbyfrequentmixedinfectionsandloosetransmissionbottlenecks AT richardcallen transmissionofequineinfluenzavirusduringanoutbreakischaracterizedbyfrequentmixedinfectionsandloosetransmissionbottlenecks AT marcbaguelin transmissionofequineinfluenzavirusduringanoutbreakischaracterizedbyfrequentmixedinfectionsandloosetransmissionbottlenecks AT katiehampson transmissionofequineinfluenzavirusduringanoutbreakischaracterizedbyfrequentmixedinfectionsandloosetransmissionbottlenecks AT gregoryjbaillie transmissionofequineinfluenzavirusduringanoutbreakischaracterizedbyfrequentmixedinfectionsandloosetransmissionbottlenecks AT debraelton transmissionofequineinfluenzavirusduringanoutbreakischaracterizedbyfrequentmixedinfectionsandloosetransmissionbottlenecks AT jrichardnewton transmissionofequineinfluenzavirusduringanoutbreakischaracterizedbyfrequentmixedinfectionsandloosetransmissionbottlenecks AT paulkellam transmissionofequineinfluenzavirusduringanoutbreakischaracterizedbyfrequentmixedinfectionsandloosetransmissionbottlenecks AT jameslnwood transmissionofequineinfluenzavirusduringanoutbreakischaracterizedbyfrequentmixedinfectionsandloosetransmissionbottlenecks AT edwardcholmes transmissionofequineinfluenzavirusduringanoutbreakischaracterizedbyfrequentmixedinfectionsandloosetransmissionbottlenecks AT pablormurcia transmissionofequineinfluenzavirusduringanoutbreakischaracterizedbyfrequentmixedinfectionsandloosetransmissionbottlenecks
_version_	1718424522159292416

Transmission of equine influenza virus during an outbreak is characterized by frequent mixed infections and loose transmission bottlenecks.

Ejemplares similares