The proteomics analysis of the effects of Zhishi Rhubarb soup on ischaemic stroke

Abstract Background Stroke has always been a major threat worldwide but is most severe in China, with 2.5 million new stroke cases each year and 7.5 million stroke survivors, placing a heavy burden on the social and national health care systems. Zhishi Rhubarb Soup (ZRS) is a traditional Chinese med...

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Autores principales:	Jing-Hua Zhang, Yue-Jia Shao, Zhen Hui, Su-Lei Wang, Chi Huang, Yang Zhao
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Ischaemic stroke Neurogenesis Vitamin transport Immune response Inflammation Cytology QH573-671
Acceso en línea:	https://doaj.org/article/1a6606b247a64f7982fa52d332fe2de9
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spelling	oai:doaj.org-article:1a6606b247a64f7982fa52d332fe2de92021-11-14T12:06:28ZThe proteomics analysis of the effects of Zhishi Rhubarb soup on ischaemic stroke10.1186/s12953-021-00181-z1477-5956https://doaj.org/article/1a6606b247a64f7982fa52d332fe2de92021-11-01T00:00:00Zhttps://doi.org/10.1186/s12953-021-00181-zhttps://doaj.org/toc/1477-5956Abstract Background Stroke has always been a major threat worldwide but is most severe in China, with 2.5 million new stroke cases each year and 7.5 million stroke survivors, placing a heavy burden on the social and national health care systems. Zhishi Rhubarb Soup (ZRS) is a traditional Chinese medicine (TCM) that has been used clinically for many years in China. To explore the potential mechanism of ZRS in the treatment of stroke, liquid chromatography with mass spectrometry (LC–MS) was performed. Methods In this study, a quantitative proteomic method with LC–MS was used to analyse the proteomic differences between MACO samples treated with ZRS and those without ZRS treatment. Results Liquid chromatography with mass spectrometry (LC–MS) analysis led to the identification of 35,006 peptides, with 5160.0 proteins identified and 4094.0 quantified. Significantly differentially expressed proteins were identified through data analysis, and the difference was found to be more than 1.2 times (P < 0.05). The Gene Ontology (GO) analysis provided a summary of the dysregulated protein expression in the biological process (BP), cell component (CC), and molecular function (MF) categories. Proteins related to brain repair, including BDNF, IL-10, IL-6, and TGF-β, were found to change significantly, partially demonstrating the effectiveness of ZRS to attenuate tissue injury. Conclusion In this study, LC–MS/MS was performed to assess the effects of ZRS on differentially expressed proteins in rats with cerebral infarction. These promising results could help to improve the understanding of the effects of drugs on stroke.Jing-Hua ZhangYue-Jia ShaoZhen HuiSu-Lei WangChi HuangYang ZhaoBMCarticleIschaemic strokeNeurogenesisVitamin transportImmune responseInflammationCytologyQH573-671ENProteome Science, Vol 19, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ischaemic stroke Neurogenesis Vitamin transport Immune response Inflammation Cytology QH573-671
spellingShingle	Ischaemic stroke Neurogenesis Vitamin transport Immune response Inflammation Cytology QH573-671 Jing-Hua Zhang Yue-Jia Shao Zhen Hui Su-Lei Wang Chi Huang Yang Zhao The proteomics analysis of the effects of Zhishi Rhubarb soup on ischaemic stroke
description	Abstract Background Stroke has always been a major threat worldwide but is most severe in China, with 2.5 million new stroke cases each year and 7.5 million stroke survivors, placing a heavy burden on the social and national health care systems. Zhishi Rhubarb Soup (ZRS) is a traditional Chinese medicine (TCM) that has been used clinically for many years in China. To explore the potential mechanism of ZRS in the treatment of stroke, liquid chromatography with mass spectrometry (LC–MS) was performed. Methods In this study, a quantitative proteomic method with LC–MS was used to analyse the proteomic differences between MACO samples treated with ZRS and those without ZRS treatment. Results Liquid chromatography with mass spectrometry (LC–MS) analysis led to the identification of 35,006 peptides, with 5160.0 proteins identified and 4094.0 quantified. Significantly differentially expressed proteins were identified through data analysis, and the difference was found to be more than 1.2 times (P < 0.05). The Gene Ontology (GO) analysis provided a summary of the dysregulated protein expression in the biological process (BP), cell component (CC), and molecular function (MF) categories. Proteins related to brain repair, including BDNF, IL-10, IL-6, and TGF-β, were found to change significantly, partially demonstrating the effectiveness of ZRS to attenuate tissue injury. Conclusion In this study, LC–MS/MS was performed to assess the effects of ZRS on differentially expressed proteins in rats with cerebral infarction. These promising results could help to improve the understanding of the effects of drugs on stroke.
format	article
author	Jing-Hua Zhang Yue-Jia Shao Zhen Hui Su-Lei Wang Chi Huang Yang Zhao
author_facet	Jing-Hua Zhang Yue-Jia Shao Zhen Hui Su-Lei Wang Chi Huang Yang Zhao
author_sort	Jing-Hua Zhang
title	The proteomics analysis of the effects of Zhishi Rhubarb soup on ischaemic stroke
title_short	The proteomics analysis of the effects of Zhishi Rhubarb soup on ischaemic stroke
title_full	The proteomics analysis of the effects of Zhishi Rhubarb soup on ischaemic stroke
title_fullStr	The proteomics analysis of the effects of Zhishi Rhubarb soup on ischaemic stroke
title_full_unstemmed	The proteomics analysis of the effects of Zhishi Rhubarb soup on ischaemic stroke
title_sort	proteomics analysis of the effects of zhishi rhubarb soup on ischaemic stroke
publisher	BMC
publishDate	2021
url	https://doaj.org/article/1a6606b247a64f7982fa52d332fe2de9
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The proteomics analysis of the effects of Zhishi Rhubarb soup on ischaemic stroke

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