Tracking single particles for hours via continuous DNA-mediated fluorophore exchange

The length of single-particle tracking experiments are limited due to photobleaching. Here the authors achieve long-term single-particle tracking with continuous fluorophore exchange in DNA-PAINT and use this to observe DNA origami on lipid bilayers for tens of minutes.

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Autores principales: Florian Stehr, Johannes Stein, Julian Bauer, Christian Niederauer, Ralf Jungmann, Kristina Ganzinger, Petra Schwille
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/1b2f7a94852a468ebfe04d98c2bcdb3f
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