Tracking single particles for hours via continuous DNA-mediated fluorophore exchange

The length of single-particle tracking experiments are limited due to photobleaching. Here the authors achieve long-term single-particle tracking with continuous fluorophore exchange in DNA-PAINT and use this to observe DNA origami on lipid bilayers for tens of minutes.

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Autores principales:	Florian Stehr, Johannes Stein, Julian Bauer, Christian Niederauer, Ralf Jungmann, Kristina Ganzinger, Petra Schwille
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/1b2f7a94852a468ebfe04d98c2bcdb3f
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spelling	oai:doaj.org-article:1b2f7a94852a468ebfe04d98c2bcdb3f2021-12-02T17:57:16ZTracking single particles for hours via continuous DNA-mediated fluorophore exchange10.1038/s41467-021-24223-42041-1723https://doaj.org/article/1b2f7a94852a468ebfe04d98c2bcdb3f2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-24223-4https://doaj.org/toc/2041-1723The length of single-particle tracking experiments are limited due to photobleaching. Here the authors achieve long-term single-particle tracking with continuous fluorophore exchange in DNA-PAINT and use this to observe DNA origami on lipid bilayers for tens of minutes.Florian StehrJohannes SteinJulian BauerChristian NiederauerRalf JungmannKristina GanzingerPetra SchwilleNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Florian Stehr Johannes Stein Julian Bauer Christian Niederauer Ralf Jungmann Kristina Ganzinger Petra Schwille Tracking single particles for hours via continuous DNA-mediated fluorophore exchange
description	The length of single-particle tracking experiments are limited due to photobleaching. Here the authors achieve long-term single-particle tracking with continuous fluorophore exchange in DNA-PAINT and use this to observe DNA origami on lipid bilayers for tens of minutes.
format	article
author	Florian Stehr Johannes Stein Julian Bauer Christian Niederauer Ralf Jungmann Kristina Ganzinger Petra Schwille
author_facet	Florian Stehr Johannes Stein Julian Bauer Christian Niederauer Ralf Jungmann Kristina Ganzinger Petra Schwille
author_sort	Florian Stehr
title	Tracking single particles for hours via continuous DNA-mediated fluorophore exchange
title_short	Tracking single particles for hours via continuous DNA-mediated fluorophore exchange
title_full	Tracking single particles for hours via continuous DNA-mediated fluorophore exchange
title_fullStr	Tracking single particles for hours via continuous DNA-mediated fluorophore exchange
title_full_unstemmed	Tracking single particles for hours via continuous DNA-mediated fluorophore exchange
title_sort	tracking single particles for hours via continuous dna-mediated fluorophore exchange
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1b2f7a94852a468ebfe04d98c2bcdb3f
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Tracking single particles for hours via continuous DNA-mediated fluorophore exchange

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