The smfBox is an open-source platform for single-molecule FRET

Broad uptake of smFRET has been hindered by high instrument costs and a lack of open-source hardware and acquisition software. Here, the authors present the smfBox, a cost-effective open-source platform capable of measuring precise FRET efficiencies between dyes on freely diffusing single molecules.

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Autores principales: Benjamin Ambrose, James M. Baxter, John Cully, Matthew Willmott, Elliot M. Steele, Benji C. Bateman, Marisa L. Martin-Fernandez, Ashley Cadby, Jonathan Shewring, Marleen Aaldering, Timothy D. Craggs
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Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/1b8fcae7f02c497fbb6bd0987c70a694
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spelling oai:doaj.org-article:1b8fcae7f02c497fbb6bd0987c70a6942021-12-02T17:31:25ZThe smfBox is an open-source platform for single-molecule FRET10.1038/s41467-020-19468-42041-1723https://doaj.org/article/1b8fcae7f02c497fbb6bd0987c70a6942020-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-19468-4https://doaj.org/toc/2041-1723Broad uptake of smFRET has been hindered by high instrument costs and a lack of open-source hardware and acquisition software. Here, the authors present the smfBox, a cost-effective open-source platform capable of measuring precise FRET efficiencies between dyes on freely diffusing single molecules.Benjamin AmbroseJames M. BaxterJohn CullyMatthew WillmottElliot M. SteeleBenji C. BatemanMarisa L. Martin-FernandezAshley CadbyJonathan ShewringMarleen AalderingTimothy D. CraggsNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-6 (2020)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Benjamin Ambrose
James M. Baxter
John Cully
Matthew Willmott
Elliot M. Steele
Benji C. Bateman
Marisa L. Martin-Fernandez
Ashley Cadby
Jonathan Shewring
Marleen Aaldering
Timothy D. Craggs
The smfBox is an open-source platform for single-molecule FRET
description Broad uptake of smFRET has been hindered by high instrument costs and a lack of open-source hardware and acquisition software. Here, the authors present the smfBox, a cost-effective open-source platform capable of measuring precise FRET efficiencies between dyes on freely diffusing single molecules.
format article
author Benjamin Ambrose
James M. Baxter
John Cully
Matthew Willmott
Elliot M. Steele
Benji C. Bateman
Marisa L. Martin-Fernandez
Ashley Cadby
Jonathan Shewring
Marleen Aaldering
Timothy D. Craggs
author_facet Benjamin Ambrose
James M. Baxter
John Cully
Matthew Willmott
Elliot M. Steele
Benji C. Bateman
Marisa L. Martin-Fernandez
Ashley Cadby
Jonathan Shewring
Marleen Aaldering
Timothy D. Craggs
author_sort Benjamin Ambrose
title The smfBox is an open-source platform for single-molecule FRET
title_short The smfBox is an open-source platform for single-molecule FRET
title_full The smfBox is an open-source platform for single-molecule FRET
title_fullStr The smfBox is an open-source platform for single-molecule FRET
title_full_unstemmed The smfBox is an open-source platform for single-molecule FRET
title_sort smfbox is an open-source platform for single-molecule fret
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/1b8fcae7f02c497fbb6bd0987c70a694
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