Multiplex single-cell visualization of nucleic acids and protein during HIV infection

Multiplex single-cell visualization of nucleic acids and protein during HIV infection

Technical limitations in simultaneous microscopic visualization of HIV transcription from individual integration sites have curtailed progress in the field. Here the authors report a branched DNA in situ hybridization method for direct single-cell visualization of HIV DNA, RNA, and protein.

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Autores principales: Maritza Puray-Chavez, Philip R. Tedbury, Andrew D. Huber, Obiaara B. Ukah, Vincent Yapo, Dandan Liu, Juan Ji, Jennifer J. Wolf, Alan N. Engelman, Stefan G. Sarafianos
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Science
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Acceso en línea:https://doaj.org/article/1c1971c8f6e44462b70f027522cf0fa9
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https://doaj.org/article/1c1971c8f6e44462b70f027522cf0fa9

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