Multiplex single-cell visualization of nucleic acids and protein during HIV infection

Technical limitations in simultaneous microscopic visualization of HIV transcription from individual integration sites have curtailed progress in the field. Here the authors report a branched DNA in situ hybridization method for direct single-cell visualization of HIV DNA, RNA, and protein.

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Autores principales:	Maritza Puray-Chavez, Philip R. Tedbury, Andrew D. Huber, Obiaara B. Ukah, Vincent Yapo, Dandan Liu, Juan Ji, Jennifer J. Wolf, Alan N. Engelman, Stefan G. Sarafianos
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/1c1971c8f6e44462b70f027522cf0fa9
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spelling	oai:doaj.org-article:1c1971c8f6e44462b70f027522cf0fa92021-12-02T17:06:01ZMultiplex single-cell visualization of nucleic acids and protein during HIV infection10.1038/s41467-017-01693-z2041-1723https://doaj.org/article/1c1971c8f6e44462b70f027522cf0fa92017-12-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-01693-zhttps://doaj.org/toc/2041-1723Technical limitations in simultaneous microscopic visualization of HIV transcription from individual integration sites have curtailed progress in the field. Here the authors report a branched DNA in situ hybridization method for direct single-cell visualization of HIV DNA, RNA, and protein.Maritza Puray-ChavezPhilip R. TedburyAndrew D. HuberObiaara B. UkahVincent YapoDandan LiuJuan JiJennifer J. WolfAlan N. EngelmanStefan G. SarafianosNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Maritza Puray-Chavez Philip R. Tedbury Andrew D. Huber Obiaara B. Ukah Vincent Yapo Dandan Liu Juan Ji Jennifer J. Wolf Alan N. Engelman Stefan G. Sarafianos Multiplex single-cell visualization of nucleic acids and protein during HIV infection
description	Technical limitations in simultaneous microscopic visualization of HIV transcription from individual integration sites have curtailed progress in the field. Here the authors report a branched DNA in situ hybridization method for direct single-cell visualization of HIV DNA, RNA, and protein.
format	article
author	Maritza Puray-Chavez Philip R. Tedbury Andrew D. Huber Obiaara B. Ukah Vincent Yapo Dandan Liu Juan Ji Jennifer J. Wolf Alan N. Engelman Stefan G. Sarafianos
author_facet	Maritza Puray-Chavez Philip R. Tedbury Andrew D. Huber Obiaara B. Ukah Vincent Yapo Dandan Liu Juan Ji Jennifer J. Wolf Alan N. Engelman Stefan G. Sarafianos
author_sort	Maritza Puray-Chavez
title	Multiplex single-cell visualization of nucleic acids and protein during HIV infection
title_short	Multiplex single-cell visualization of nucleic acids and protein during HIV infection
title_full	Multiplex single-cell visualization of nucleic acids and protein during HIV infection
title_fullStr	Multiplex single-cell visualization of nucleic acids and protein during HIV infection
title_full_unstemmed	Multiplex single-cell visualization of nucleic acids and protein during HIV infection
title_sort	multiplex single-cell visualization of nucleic acids and protein during hiv infection
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1c1971c8f6e44462b70f027522cf0fa9
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Multiplex single-cell visualization of nucleic acids and protein during HIV infection

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