Isolation and characterization of maize PMP3 genes involved in salt stress tolerance.

Plasma membrane protein 3 (PMP3), a class of small hydrophobic polypeptides with high sequence similarity, is responsible for salt, drought, cold, and abscisic acid. These small hydrophobic ploypeptides play important roles in maintenance of ion homeostasis. In this study, eight ZmPMP3 genes were cl...

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Main Authors:	Jing Fu, Deng-Feng Zhang, Ying-Hui Liu, Sheng Ying, Yun-Su Shi, Yan-Chun Song, Yu Li, Tian-Yu Wang
Format:	article
Language:	EN
Published:	Public Library of Science (PLoS) 2012
Subjects:	Medicine R Science Q
Online Access:	https://doaj.org/article/1c8b6b99a3054fbb8c1a7b420e2f649c
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spelling	oai:doaj.org-article:1c8b6b99a3054fbb8c1a7b420e2f649c2021-11-18T07:28:28ZIsolation and characterization of maize PMP3 genes involved in salt stress tolerance.1932-620310.1371/journal.pone.0031101https://doaj.org/article/1c8b6b99a3054fbb8c1a7b420e2f649c2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22348040/?tool=EBIhttps://doaj.org/toc/1932-6203Plasma membrane protein 3 (PMP3), a class of small hydrophobic polypeptides with high sequence similarity, is responsible for salt, drought, cold, and abscisic acid. These small hydrophobic ploypeptides play important roles in maintenance of ion homeostasis. In this study, eight ZmPMP3 genes were cloned from maize and responsive to salt, drought, cold and abscisic acid. The eight ZmPMP3s were membrane proteins and their sequences in trans-membrane regions were highly conserved. Phylogenetic analysis showed that they were categorized into three groups. All members of group II were responsive to ABA. Functional complementation showed that with the exception of ZmPMP3-6, all were capable of maintaining membrane potential, which in turn allows for regulation of intracellular ion homeostasis. This process was independent of the presence of Ca(2+). Lastly, over-expression of ZmPMP3-1 enhanced growth of transgenic Arabidopsis under salt condition. Through expression analysis of deduced downstream genes in transgenic plants, expression levels of three ion transporter genes and four important antioxidant genes in ROS scavenging system were increased significantly in transgenic plants during salt stress. This tolerance was likely achieved through diminishing oxidative stress due to the possibility of ZmPMP3-1's involvement in regulation of ion homeostasis, and suggests that the modulation of these conserved small hydrophobic polypeptides could be an effective way to improve salt tolerance in plants.Jing FuDeng-Feng ZhangYing-Hui LiuSheng YingYun-Su ShiYan-Chun SongYu LiTian-Yu WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e31101 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jing Fu Deng-Feng Zhang Ying-Hui Liu Sheng Ying Yun-Su Shi Yan-Chun Song Yu Li Tian-Yu Wang Isolation and characterization of maize PMP3 genes involved in salt stress tolerance.
description	Plasma membrane protein 3 (PMP3), a class of small hydrophobic polypeptides with high sequence similarity, is responsible for salt, drought, cold, and abscisic acid. These small hydrophobic ploypeptides play important roles in maintenance of ion homeostasis. In this study, eight ZmPMP3 genes were cloned from maize and responsive to salt, drought, cold and abscisic acid. The eight ZmPMP3s were membrane proteins and their sequences in trans-membrane regions were highly conserved. Phylogenetic analysis showed that they were categorized into three groups. All members of group II were responsive to ABA. Functional complementation showed that with the exception of ZmPMP3-6, all were capable of maintaining membrane potential, which in turn allows for regulation of intracellular ion homeostasis. This process was independent of the presence of Ca(2+). Lastly, over-expression of ZmPMP3-1 enhanced growth of transgenic Arabidopsis under salt condition. Through expression analysis of deduced downstream genes in transgenic plants, expression levels of three ion transporter genes and four important antioxidant genes in ROS scavenging system were increased significantly in transgenic plants during salt stress. This tolerance was likely achieved through diminishing oxidative stress due to the possibility of ZmPMP3-1's involvement in regulation of ion homeostasis, and suggests that the modulation of these conserved small hydrophobic polypeptides could be an effective way to improve salt tolerance in plants.
format	article
author	Jing Fu Deng-Feng Zhang Ying-Hui Liu Sheng Ying Yun-Su Shi Yan-Chun Song Yu Li Tian-Yu Wang
author_facet	Jing Fu Deng-Feng Zhang Ying-Hui Liu Sheng Ying Yun-Su Shi Yan-Chun Song Yu Li Tian-Yu Wang
author_sort	Jing Fu
title	Isolation and characterization of maize PMP3 genes involved in salt stress tolerance.
title_short	Isolation and characterization of maize PMP3 genes involved in salt stress tolerance.
title_full	Isolation and characterization of maize PMP3 genes involved in salt stress tolerance.
title_fullStr	Isolation and characterization of maize PMP3 genes involved in salt stress tolerance.
title_full_unstemmed	Isolation and characterization of maize PMP3 genes involved in salt stress tolerance.
title_sort	isolation and characterization of maize pmp3 genes involved in salt stress tolerance.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/1c8b6b99a3054fbb8c1a7b420e2f649c
work_keys_str_mv	AT jingfu isolationandcharacterizationofmaizepmp3genesinvolvedinsaltstresstolerance AT dengfengzhang isolationandcharacterizationofmaizepmp3genesinvolvedinsaltstresstolerance AT yinghuiliu isolationandcharacterizationofmaizepmp3genesinvolvedinsaltstresstolerance AT shengying isolationandcharacterizationofmaizepmp3genesinvolvedinsaltstresstolerance AT yunsushi isolationandcharacterizationofmaizepmp3genesinvolvedinsaltstresstolerance AT yanchunsong isolationandcharacterizationofmaizepmp3genesinvolvedinsaltstresstolerance AT yuli isolationandcharacterizationofmaizepmp3genesinvolvedinsaltstresstolerance AT tianyuwang isolationandcharacterizationofmaizepmp3genesinvolvedinsaltstresstolerance
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Isolation and characterization of maize PMP3 genes involved in salt stress tolerance.

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