Molecular identification of pathogenic bacteria causing foodborne disease in Caulerpa racemosa

Caulerpa racemosa is a green algae consumed by people in northern coastal areas. C. racemosa has a habitat attached to the shallow seabed. C. racemosa usualy consumed fresh without any cooking process so that the contamination of microorganisms can be eaten. Molecular identification using 16S rRNA i...

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Autores principales:	Aprilia Indra Kartika, Meutia Srikandi Fitria, Vanny Oktaviola
Formato:	article
Lenguaje:	ID
Publicado:	Poltekkes Kemenkes Yogyakarta 2021
Materias:	bacteria identification caulerpa racemose foodborne disease Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1ca1ae281ddd4919a5f94be97bc798af
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spelling	oai:doaj.org-article:1ca1ae281ddd4919a5f94be97bc798af2021-11-13T13:29:49ZMolecular identification of pathogenic bacteria causing foodborne disease in Caulerpa racemosa2338-56342580-019110.29238/teknolabjournal.v10i1.276https://doaj.org/article/1ca1ae281ddd4919a5f94be97bc798af2021-08-01T00:00:00Zhttps://teknolabjournal.com/index.php/Jtl/article/view/276https://doaj.org/toc/2338-5634https://doaj.org/toc/2580-0191Caulerpa racemosa is a green algae consumed by people in northern coastal areas. C. racemosa has a habitat attached to the shallow seabed. C. racemosa usualy consumed fresh without any cooking process so that the contamination of microorganisms can be eaten. Molecular identification using 16S rRNA is needed to determine the type of bacterial contaminants in C. racemosa. The isolates of C. racemosa were cultured in HIA, BAP, and BHI media. Bacteria from BHI media were isolated by DNA, PCR for 16S rRNA gene, and sequencing. Bacteria isolate C. racemosa was found to have the α-hemolytic ability in BAP media. The sequencing analysis showed that the three bacterial colonies of C. racemosa isolate had high similarity with V. parahemolyticus, Caldalkalibacillus mannanilyticus, and Exiguobacterium profundum.Aprilia Indra KartikaMeutia Srikandi FitriaVanny OktaviolaPoltekkes Kemenkes Yogyakartaarticlebacteria identificationcaulerpa racemosefoodborne diseaseMedicine (General)R5-920IDJurnal Teknologi Laboratorium, Vol 10, Iss 1, Pp 31-39 (2021)
institution	DOAJ
collection	DOAJ
language	ID
topic	bacteria identification caulerpa racemose foodborne disease Medicine (General) R5-920
spellingShingle	bacteria identification caulerpa racemose foodborne disease Medicine (General) R5-920 Aprilia Indra Kartika Meutia Srikandi Fitria Vanny Oktaviola Molecular identification of pathogenic bacteria causing foodborne disease in Caulerpa racemosa
description	Caulerpa racemosa is a green algae consumed by people in northern coastal areas. C. racemosa has a habitat attached to the shallow seabed. C. racemosa usualy consumed fresh without any cooking process so that the contamination of microorganisms can be eaten. Molecular identification using 16S rRNA is needed to determine the type of bacterial contaminants in C. racemosa. The isolates of C. racemosa were cultured in HIA, BAP, and BHI media. Bacteria from BHI media were isolated by DNA, PCR for 16S rRNA gene, and sequencing. Bacteria isolate C. racemosa was found to have the α-hemolytic ability in BAP media. The sequencing analysis showed that the three bacterial colonies of C. racemosa isolate had high similarity with V. parahemolyticus, Caldalkalibacillus mannanilyticus, and Exiguobacterium profundum.
format	article
author	Aprilia Indra Kartika Meutia Srikandi Fitria Vanny Oktaviola
author_facet	Aprilia Indra Kartika Meutia Srikandi Fitria Vanny Oktaviola
author_sort	Aprilia Indra Kartika
title	Molecular identification of pathogenic bacteria causing foodborne disease in Caulerpa racemosa
title_short	Molecular identification of pathogenic bacteria causing foodborne disease in Caulerpa racemosa
title_full	Molecular identification of pathogenic bacteria causing foodborne disease in Caulerpa racemosa
title_fullStr	Molecular identification of pathogenic bacteria causing foodborne disease in Caulerpa racemosa
title_full_unstemmed	Molecular identification of pathogenic bacteria causing foodborne disease in Caulerpa racemosa
title_sort	molecular identification of pathogenic bacteria causing foodborne disease in caulerpa racemosa
publisher	Poltekkes Kemenkes Yogyakarta
publishDate	2021
url	https://doaj.org/article/1ca1ae281ddd4919a5f94be97bc798af
work_keys_str_mv	AT apriliaindrakartika molecularidentificationofpathogenicbacteriacausingfoodbornediseaseincaulerparacemosa AT meutiasrikandifitria molecularidentificationofpathogenicbacteriacausingfoodbornediseaseincaulerparacemosa AT vannyoktaviola molecularidentificationofpathogenicbacteriacausingfoodbornediseaseincaulerparacemosa
_version_	1718430240421707776

Molecular identification of pathogenic bacteria causing foodborne disease in Caulerpa racemosa

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