Characterizing a thermostable Cas9 for bacterial genome editing and silencing

CRISPR-Cas9 genome engineering tools have found wide application in a range of species, however they are unsuitable for applications at elevated temperatures. Here the authors characterise ThermoCas9 from which is functional from 20°C to 70°C.

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Auteurs principaux: Ioannis Mougiakos, Prarthana Mohanraju, Elleke F. Bosma, Valentijn Vrouwe, Max Finger Bou, Mihris I. S. Naduthodi, Alex Gussak, Rudolf B. L. Brinkman, Richard van Kranenburg, John van der Oost
Format: article
Langue:EN
Publié: Nature Portfolio 2017
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Accès en ligne:https://doaj.org/article/1d2d2fee1e3d44708bd22d9330e108c2
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