Characterizing a thermostable Cas9 for bacterial genome editing and silencing

CRISPR-Cas9 genome engineering tools have found wide application in a range of species, however they are unsuitable for applications at elevated temperatures. Here the authors characterise ThermoCas9 from which is functional from 20°C to 70°C.

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Autores principales:	Ioannis Mougiakos, Prarthana Mohanraju, Elleke F. Bosma, Valentijn Vrouwe, Max Finger Bou, Mihris I. S. Naduthodi, Alex Gussak, Rudolf B. L. Brinkman, Richard van Kranenburg, John van der Oost
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/1d2d2fee1e3d44708bd22d9330e108c2
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spelling	oai:doaj.org-article:1d2d2fee1e3d44708bd22d9330e108c22021-12-02T14:40:38ZCharacterizing a thermostable Cas9 for bacterial genome editing and silencing10.1038/s41467-017-01591-42041-1723https://doaj.org/article/1d2d2fee1e3d44708bd22d9330e108c22017-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-01591-4https://doaj.org/toc/2041-1723CRISPR-Cas9 genome engineering tools have found wide application in a range of species, however they are unsuitable for applications at elevated temperatures. Here the authors characterise ThermoCas9 from which is functional from 20°C to 70°C.Ioannis MougiakosPrarthana MohanrajuElleke F. BosmaValentijn VrouweMax Finger BouMihris I. S. NaduthodiAlex GussakRudolf B. L. BrinkmanRichard van KranenburgJohn van der OostNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Ioannis Mougiakos Prarthana Mohanraju Elleke F. Bosma Valentijn Vrouwe Max Finger Bou Mihris I. S. Naduthodi Alex Gussak Rudolf B. L. Brinkman Richard van Kranenburg John van der Oost Characterizing a thermostable Cas9 for bacterial genome editing and silencing
description	CRISPR-Cas9 genome engineering tools have found wide application in a range of species, however they are unsuitable for applications at elevated temperatures. Here the authors characterise ThermoCas9 from which is functional from 20°C to 70°C.
format	article
author	Ioannis Mougiakos Prarthana Mohanraju Elleke F. Bosma Valentijn Vrouwe Max Finger Bou Mihris I. S. Naduthodi Alex Gussak Rudolf B. L. Brinkman Richard van Kranenburg John van der Oost
author_facet	Ioannis Mougiakos Prarthana Mohanraju Elleke F. Bosma Valentijn Vrouwe Max Finger Bou Mihris I. S. Naduthodi Alex Gussak Rudolf B. L. Brinkman Richard van Kranenburg John van der Oost
author_sort	Ioannis Mougiakos
title	Characterizing a thermostable Cas9 for bacterial genome editing and silencing
title_short	Characterizing a thermostable Cas9 for bacterial genome editing and silencing
title_full	Characterizing a thermostable Cas9 for bacterial genome editing and silencing
title_fullStr	Characterizing a thermostable Cas9 for bacterial genome editing and silencing
title_full_unstemmed	Characterizing a thermostable Cas9 for bacterial genome editing and silencing
title_sort	characterizing a thermostable cas9 for bacterial genome editing and silencing
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1d2d2fee1e3d44708bd22d9330e108c2
work_keys_str_mv	AT ioannismougiakos characterizingathermostablecas9forbacterialgenomeeditingandsilencing AT prarthanamohanraju characterizingathermostablecas9forbacterialgenomeeditingandsilencing AT ellekefbosma characterizingathermostablecas9forbacterialgenomeeditingandsilencing AT valentijnvrouwe characterizingathermostablecas9forbacterialgenomeeditingandsilencing AT maxfingerbou characterizingathermostablecas9forbacterialgenomeeditingandsilencing AT mihrisisnaduthodi characterizingathermostablecas9forbacterialgenomeeditingandsilencing AT alexgussak characterizingathermostablecas9forbacterialgenomeeditingandsilencing AT rudolfblbrinkman characterizingathermostablecas9forbacterialgenomeeditingandsilencing AT richardvankranenburg characterizingathermostablecas9forbacterialgenomeeditingandsilencing AT johnvanderoost characterizingathermostablecas9forbacterialgenomeeditingandsilencing
_version_	1718390159942090752

Characterizing a thermostable Cas9 for bacterial genome editing and silencing

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