Characterizing a thermostable Cas9 for bacterial genome editing and silencing

Characterizing a thermostable Cas9 for bacterial genome editing and silencing

CRISPR-Cas9 genome engineering tools have found wide application in a range of species, however they are unsuitable for applications at elevated temperatures. Here the authors characterise ThermoCas9 from which is functional from 20°C to 70°C.

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Detalles Bibliográficos
Autores principales: Ioannis Mougiakos, Prarthana Mohanraju, Elleke F. Bosma, Valentijn Vrouwe, Max Finger Bou, Mihris I. S. Naduthodi, Alex Gussak, Rudolf B. L. Brinkman, Richard van Kranenburg, John van der Oost
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
Materias:
Science
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Acceso en línea:https://doaj.org/article/1d2d2fee1e3d44708bd22d9330e108c2
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