Redesign of ultrasensitive and robust RecA gene circuit to sense DNA damage

Summary SOS box of the recA promoter, PVRecA from Vibrio natriegens was characterized, cloned and expressed in a probiotic strain E. coli Nissle 1917. This promoter was then rationally engineered according to predicted interactions between LexA repressor and PVRecA. The redesigned PVRecA‐AT promoter...

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Autores principales: Jack X. Chen, Boon Lim, Harrison Steel, Yizhi Song, Mengmeng Ji, Wei E. Huang
Formato: article
Lenguaje:EN
Publicado: Wiley 2021
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Acceso en línea:https://doaj.org/article/1d917eaa409b4fd6825382f017ee98bb
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