Redesign of ultrasensitive and robust RecA gene circuit to sense DNA damage
Summary SOS box of the recA promoter, PVRecA from Vibrio natriegens was characterized, cloned and expressed in a probiotic strain E. coli Nissle 1917. This promoter was then rationally engineered according to predicted interactions between LexA repressor and PVRecA. The redesigned PVRecA‐AT promoter...
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Autores principales: | Jack X. Chen, Boon Lim, Harrison Steel, Yizhi Song, Mengmeng Ji, Wei E. Huang |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Wiley
2021
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Materias: | |
Acceso en línea: | https://doaj.org/article/1d917eaa409b4fd6825382f017ee98bb |
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