A robust gene-stacking method utilizing yeast assembly for plant synthetic biology

Plant synthetic biology offers the potential to re-engineer crops, but requires efficient methods to prepare constructs for transformation. Here Shih et al. develop jStack, a method that utilizes yeast homologous recombination and a library of DNA parts, to efficiently assemble plant transformation...

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Autores principales: Patrick M. Shih, Khanh Vuu, Nasim Mansoori, Leïla Ayad, Katherine B. Louie, Benjamin P. Bowen, Trent R. Northen, Dominique Loqué
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/1e118fd79def48888da062b30e671cf4
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