A robust gene-stacking method utilizing yeast assembly for plant synthetic biology

Plant synthetic biology offers the potential to re-engineer crops, but requires efficient methods to prepare constructs for transformation. Here Shih et al. develop jStack, a method that utilizes yeast homologous recombination and a library of DNA parts, to efficiently assemble plant transformation...

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Autores principales:	Patrick M. Shih, Khanh Vuu, Nasim Mansoori, Leïla Ayad, Katherine B. Louie, Benjamin P. Bowen, Trent R. Northen, Dominique Loqué
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/1e118fd79def48888da062b30e671cf4
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spelling	oai:doaj.org-article:1e118fd79def48888da062b30e671cf42021-12-02T16:56:45ZA robust gene-stacking method utilizing yeast assembly for plant synthetic biology10.1038/ncomms132152041-1723https://doaj.org/article/1e118fd79def48888da062b30e671cf42016-10-01T00:00:00Zhttps://doi.org/10.1038/ncomms13215https://doaj.org/toc/2041-1723Plant synthetic biology offers the potential to re-engineer crops, but requires efficient methods to prepare constructs for transformation. Here Shih et al. develop jStack, a method that utilizes yeast homologous recombination and a library of DNA parts, to efficiently assemble plant transformation vectors.Patrick M. ShihKhanh VuuNasim MansooriLeïla AyadKatherine B. LouieBenjamin P. BowenTrent R. NorthenDominique LoquéNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-8 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Patrick M. Shih Khanh Vuu Nasim Mansoori Leïla Ayad Katherine B. Louie Benjamin P. Bowen Trent R. Northen Dominique Loqué A robust gene-stacking method utilizing yeast assembly for plant synthetic biology
description	Plant synthetic biology offers the potential to re-engineer crops, but requires efficient methods to prepare constructs for transformation. Here Shih et al. develop jStack, a method that utilizes yeast homologous recombination and a library of DNA parts, to efficiently assemble plant transformation vectors.
format	article
author	Patrick M. Shih Khanh Vuu Nasim Mansoori Leïla Ayad Katherine B. Louie Benjamin P. Bowen Trent R. Northen Dominique Loqué
author_facet	Patrick M. Shih Khanh Vuu Nasim Mansoori Leïla Ayad Katherine B. Louie Benjamin P. Bowen Trent R. Northen Dominique Loqué
author_sort	Patrick M. Shih
title	A robust gene-stacking method utilizing yeast assembly for plant synthetic biology
title_short	A robust gene-stacking method utilizing yeast assembly for plant synthetic biology
title_full	A robust gene-stacking method utilizing yeast assembly for plant synthetic biology
title_fullStr	A robust gene-stacking method utilizing yeast assembly for plant synthetic biology
title_full_unstemmed	A robust gene-stacking method utilizing yeast assembly for plant synthetic biology
title_sort	robust gene-stacking method utilizing yeast assembly for plant synthetic biology
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/1e118fd79def48888da062b30e671cf4
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A robust gene-stacking method utilizing yeast assembly for plant synthetic biology

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