Metagenomic analysis and biodiversity of Lactic Acid Bacteria (LAB) on masin (fermented sauce) from Sumbawa, West Nusa Tenggara, Indonesia

Abstract. Manguntungi B, Saputri DS, Mustopa AZ, Ekawati N, Afgani CA, Sari AP, Triratna L, Sukmarini L, Fatimah, Kusmiran A, Yanti S, Irawan S, Prasetyo MD, Fidien KA. 2020. Metagenomic analysis and biodiversity of Lactic Acid Bacteria (LAB) on masin (fermented sauce) from Sumbawa, West Nusa Tengga...

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Autores principales:	Baso Manguntungi, Dinar Saputri, Apon Mustopa, Nurlaili Ekawati, Chairul Afgani, Arlindah Sari, Lita Triratna, Linda Sukmarini, Fatimah FATIMAH, Amirin Kusmiran, SAHRI YANTI, Shasmita Irawan, Muhammad Prasetyo, Khadijah ALLIYA Fidien
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	biodiversity, lab, masin, metagenomics Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1e24c73a6df44e1b88d9403e19fbd812
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spelling	oai:doaj.org-article:1e24c73a6df44e1b88d9403e19fbd8122021-11-22T00:40:51ZMetagenomic analysis and biodiversity of Lactic Acid Bacteria (LAB) on masin (fermented sauce) from Sumbawa, West Nusa Tenggara, Indonesia1412-033X2085-472210.13057/biodiv/d210752https://doaj.org/article/1e24c73a6df44e1b88d9403e19fbd8122020-06-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5783https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Manguntungi B, Saputri DS, Mustopa AZ, Ekawati N, Afgani CA, Sari AP, Triratna L, Sukmarini L, Fatimah, Kusmiran A, Yanti S, Irawan S, Prasetyo MD, Fidien KA. 2020. Metagenomic analysis and biodiversity of Lactic Acid Bacteria (LAB) on masin (fermented sauce) from Sumbawa, West Nusa Tenggara, Indonesia. Biodiversitas 21: 3287-3293. Masin is a spontaneously fermented sauce from Sumbawa, West Nusa Tenggara Indonesia that is made of shrimp paste, chili, turmeric flower, and herbs mixed with some spices. This study aims to isolate Lactic Acid Bacteria (LAB) and analyze the metagenomic and biodiversity of its bacteria. Genome isolation for metagenomic analysis was using ZymoBIOMICSTM DNA Miniprep Kit. Sequencing analysis to identify LAB strain form masin was using 16S rRNA universal primer. Metagenomic analysis showed that relative abundance bacteria in masin for order taxon was Lactobacillales, the family taxon was Enteroccocaceae, and the genus was Tetragenoccocus. Six different groups were obtained from the phylogenetic tree analysis of 40 isolates found in masin. The representatives of each group taken were isolates number 2, 17, 11, 34, 28, and 5. Based on the results of sequencing analysis, the 6 isolates found in masin are Staphylococcus piscifermentans strain CIP103958 (Isolate Code 2), S. piscifermentans strain BULST54 (Isolate Code 17), S. piscifermentans strain SK03 (Isolate Code 11), S. piscifermentans strain ATCC 51136 (Isolate Code 34), S. piscifermentans strain PCM 2409 (Isolate Code 28) and S. piscifermentans strain PU-87 (Isolate Code 5). Through this research, it has been described a whole diversity of bacteria contained in masin, one of them is Lactic acid bacteria. The six LAB isolates that have been identified can be developed as starter candidates for masin production.Baso ManguntungiDinar SaputriApon MustopaNurlaili EkawatiChairul AfganiArlindah SariLita TriratnaLinda SukmariniFatimah FATIMAHAmirin KusmiranSAHRI YANTIShasmita IrawanMuhammad PrasetyoKhadijah ALLIYA FidienMBI & UNS Soloarticlebiodiversity, lab, masin, metagenomicsBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 7 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	biodiversity, lab, masin, metagenomics Biology (General) QH301-705.5
spellingShingle	biodiversity, lab, masin, metagenomics Biology (General) QH301-705.5 Baso Manguntungi Dinar Saputri Apon Mustopa Nurlaili Ekawati Chairul Afgani Arlindah Sari Lita Triratna Linda Sukmarini Fatimah FATIMAH Amirin Kusmiran SAHRI YANTI Shasmita Irawan Muhammad Prasetyo Khadijah ALLIYA Fidien Metagenomic analysis and biodiversity of Lactic Acid Bacteria (LAB) on masin (fermented sauce) from Sumbawa, West Nusa Tenggara, Indonesia
description	Abstract. Manguntungi B, Saputri DS, Mustopa AZ, Ekawati N, Afgani CA, Sari AP, Triratna L, Sukmarini L, Fatimah, Kusmiran A, Yanti S, Irawan S, Prasetyo MD, Fidien KA. 2020. Metagenomic analysis and biodiversity of Lactic Acid Bacteria (LAB) on masin (fermented sauce) from Sumbawa, West Nusa Tenggara, Indonesia. Biodiversitas 21: 3287-3293. Masin is a spontaneously fermented sauce from Sumbawa, West Nusa Tenggara Indonesia that is made of shrimp paste, chili, turmeric flower, and herbs mixed with some spices. This study aims to isolate Lactic Acid Bacteria (LAB) and analyze the metagenomic and biodiversity of its bacteria. Genome isolation for metagenomic analysis was using ZymoBIOMICSTM DNA Miniprep Kit. Sequencing analysis to identify LAB strain form masin was using 16S rRNA universal primer. Metagenomic analysis showed that relative abundance bacteria in masin for order taxon was Lactobacillales, the family taxon was Enteroccocaceae, and the genus was Tetragenoccocus. Six different groups were obtained from the phylogenetic tree analysis of 40 isolates found in masin. The representatives of each group taken were isolates number 2, 17, 11, 34, 28, and 5. Based on the results of sequencing analysis, the 6 isolates found in masin are Staphylococcus piscifermentans strain CIP103958 (Isolate Code 2), S. piscifermentans strain BULST54 (Isolate Code 17), S. piscifermentans strain SK03 (Isolate Code 11), S. piscifermentans strain ATCC 51136 (Isolate Code 34), S. piscifermentans strain PCM 2409 (Isolate Code 28) and S. piscifermentans strain PU-87 (Isolate Code 5). Through this research, it has been described a whole diversity of bacteria contained in masin, one of them is Lactic acid bacteria. The six LAB isolates that have been identified can be developed as starter candidates for masin production.
format	article
author	Baso Manguntungi Dinar Saputri Apon Mustopa Nurlaili Ekawati Chairul Afgani Arlindah Sari Lita Triratna Linda Sukmarini Fatimah FATIMAH Amirin Kusmiran SAHRI YANTI Shasmita Irawan Muhammad Prasetyo Khadijah ALLIYA Fidien
author_facet	Baso Manguntungi Dinar Saputri Apon Mustopa Nurlaili Ekawati Chairul Afgani Arlindah Sari Lita Triratna Linda Sukmarini Fatimah FATIMAH Amirin Kusmiran SAHRI YANTI Shasmita Irawan Muhammad Prasetyo Khadijah ALLIYA Fidien
author_sort	Baso Manguntungi
title	Metagenomic analysis and biodiversity of Lactic Acid Bacteria (LAB) on masin (fermented sauce) from Sumbawa, West Nusa Tenggara, Indonesia
title_short	Metagenomic analysis and biodiversity of Lactic Acid Bacteria (LAB) on masin (fermented sauce) from Sumbawa, West Nusa Tenggara, Indonesia
title_full	Metagenomic analysis and biodiversity of Lactic Acid Bacteria (LAB) on masin (fermented sauce) from Sumbawa, West Nusa Tenggara, Indonesia
title_fullStr	Metagenomic analysis and biodiversity of Lactic Acid Bacteria (LAB) on masin (fermented sauce) from Sumbawa, West Nusa Tenggara, Indonesia
title_full_unstemmed	Metagenomic analysis and biodiversity of Lactic Acid Bacteria (LAB) on masin (fermented sauce) from Sumbawa, West Nusa Tenggara, Indonesia
title_sort	metagenomic analysis and biodiversity of lactic acid bacteria (lab) on masin (fermented sauce) from sumbawa, west nusa tenggara, indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/1e24c73a6df44e1b88d9403e19fbd812
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Metagenomic analysis and biodiversity of Lactic Acid Bacteria (LAB) on masin (fermented sauce) from Sumbawa, West Nusa Tenggara, Indonesia

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