piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition

Prime editing is a novel genome editing technology that allows a wide range of tailored genomic alterations. Prime editing does not involve homologous recombination, but suffers from low efficacy. Here, we demonstrate piggyPrime, a transfected single-vector system based on piggyBac DNA transposition...

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Autores principales: Jonas Holst Wolff, Jakob Haldrup, Emil Aagaard Thomsen, Sofie Andersen, Jacob Giehm Mikkelsen
Formato: article
Lenguaje:EN
Publicado: Frontiers Media S.A. 2021
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Acceso en línea:https://doaj.org/article/1e9f9970af5f4f1bbb8f20886290ab8a
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