piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition

Prime editing is a novel genome editing technology that allows a wide range of tailored genomic alterations. Prime editing does not involve homologous recombination, but suffers from low efficacy. Here, we demonstrate piggyPrime, a transfected single-vector system based on piggyBac DNA transposition...

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Autores principales:	Jonas Holst Wolff, Jakob Haldrup, Emil Aagaard Thomsen, Sofie Andersen, Jacob Giehm Mikkelsen
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	prime editing CRISPR prime editing guide RNA pegRNA piggyBac DNA transposition Biotechnology TP248.13-248.65 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/1e9f9970af5f4f1bbb8f20886290ab8a
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id	oai:doaj.org-article:1e9f9970af5f4f1bbb8f20886290ab8a
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spelling	oai:doaj.org-article:1e9f9970af5f4f1bbb8f20886290ab8a2021-11-12T06:07:07ZpiggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition2673-343910.3389/fgeed.2021.786893https://doaj.org/article/1e9f9970af5f4f1bbb8f20886290ab8a2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgeed.2021.786893/fullhttps://doaj.org/toc/2673-3439Prime editing is a novel genome editing technology that allows a wide range of tailored genomic alterations. Prime editing does not involve homologous recombination, but suffers from low efficacy. Here, we demonstrate piggyPrime, a transfected single-vector system based on piggyBac DNA transposition for genomic integration of all prime editing components in human cells allowing easy and effective transgenesis with prime editing efficacies up to 100% in cell lines.Jonas Holst WolffJakob HaldrupEmil Aagaard ThomsenSofie AndersenJacob Giehm MikkelsenFrontiers Media S.A.articleprime editingCRISPRprime editing guide RNApegRNApiggyBacDNA transpositionBiotechnologyTP248.13-248.65GeneticsQH426-470ENFrontiers in Genome Editing, Vol 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	prime editing CRISPR prime editing guide RNA pegRNA piggyBac DNA transposition Biotechnology TP248.13-248.65 Genetics QH426-470
spellingShingle	prime editing CRISPR prime editing guide RNA pegRNA piggyBac DNA transposition Biotechnology TP248.13-248.65 Genetics QH426-470 Jonas Holst Wolff Jakob Haldrup Emil Aagaard Thomsen Sofie Andersen Jacob Giehm Mikkelsen piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition
description	Prime editing is a novel genome editing technology that allows a wide range of tailored genomic alterations. Prime editing does not involve homologous recombination, but suffers from low efficacy. Here, we demonstrate piggyPrime, a transfected single-vector system based on piggyBac DNA transposition for genomic integration of all prime editing components in human cells allowing easy and effective transgenesis with prime editing efficacies up to 100% in cell lines.
format	article
author	Jonas Holst Wolff Jakob Haldrup Emil Aagaard Thomsen Sofie Andersen Jacob Giehm Mikkelsen
author_facet	Jonas Holst Wolff Jakob Haldrup Emil Aagaard Thomsen Sofie Andersen Jacob Giehm Mikkelsen
author_sort	Jonas Holst Wolff
title	piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition
title_short	piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition
title_full	piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition
title_fullStr	piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition
title_full_unstemmed	piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition
title_sort	piggyprime: high-efficacy prime editing in human cells using piggybac-based dna transposition
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/1e9f9970af5f4f1bbb8f20886290ab8a
work_keys_str_mv	AT jonasholstwolff piggyprimehighefficacyprimeeditinginhumancellsusingpiggybacbaseddnatransposition AT jakobhaldrup piggyprimehighefficacyprimeeditinginhumancellsusingpiggybacbaseddnatransposition AT emilaagaardthomsen piggyprimehighefficacyprimeeditinginhumancellsusingpiggybacbaseddnatransposition AT sofieandersen piggyprimehighefficacyprimeeditinginhumancellsusingpiggybacbaseddnatransposition AT jacobgiehmmikkelsen piggyprimehighefficacyprimeeditinginhumancellsusingpiggybacbaseddnatransposition
_version_	1718431156962066432

piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition

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