Genome-wide and high-density CRISPR-Cas9 screens identify point mutations in PARP1 causing PARP inhibitor resistance
The mechanisms of PARP inhibitor (PARPi) resistance are poorly understood. Here the authors employ a CRISPR mutagenesis approach to identify PARP1 mutants causing PARPi resistance and find that PARP1 mutations are tolerated in BRCA1 mutated cells, suggesting alternative resistance mechanisms.
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Nature Portfolio
2018
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oai:doaj.org-article:1eb977c74fb546b0a7321e49be5d06432021-12-02T16:49:32ZGenome-wide and high-density CRISPR-Cas9 screens identify point mutations in PARP1 causing PARP inhibitor resistance10.1038/s41467-018-03917-22041-1723https://doaj.org/article/1eb977c74fb546b0a7321e49be5d06432018-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-03917-2https://doaj.org/toc/2041-1723The mechanisms of PARP inhibitor (PARPi) resistance are poorly understood. Here the authors employ a CRISPR mutagenesis approach to identify PARP1 mutants causing PARPi resistance and find that PARP1 mutations are tolerated in BRCA1 mutated cells, suggesting alternative resistance mechanisms.Stephen J. PettittDragomir B. KrastevInger BrandsmaAmy DréanFeifei SongRadoslav AleksandrovMaria I. HarrellMalini MenonRachel BroughJames CampbellJessica FrankumMichael RanesHelen N. PembertonRumana RafiqKerry FenwickAmanda SwainSebastian GuettlerJung-Min LeeElizabeth M. SwisherStoyno StoynovKosuke YusaAlan AshworthChristopher J. LordNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-14 (2018) |
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DOAJ |
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DOAJ |
| language |
EN |
| topic |
Science Q |
| spellingShingle |
Science Q Stephen J. Pettitt Dragomir B. Krastev Inger Brandsma Amy Dréan Feifei Song Radoslav Aleksandrov Maria I. Harrell Malini Menon Rachel Brough James Campbell Jessica Frankum Michael Ranes Helen N. Pemberton Rumana Rafiq Kerry Fenwick Amanda Swain Sebastian Guettler Jung-Min Lee Elizabeth M. Swisher Stoyno Stoynov Kosuke Yusa Alan Ashworth Christopher J. Lord Genome-wide and high-density CRISPR-Cas9 screens identify point mutations in PARP1 causing PARP inhibitor resistance |
| description |
The mechanisms of PARP inhibitor (PARPi) resistance are poorly understood. Here the authors employ a CRISPR mutagenesis approach to identify PARP1 mutants causing PARPi resistance and find that PARP1 mutations are tolerated in BRCA1 mutated cells, suggesting alternative resistance mechanisms. |
| format |
article |
| author |
Stephen J. Pettitt Dragomir B. Krastev Inger Brandsma Amy Dréan Feifei Song Radoslav Aleksandrov Maria I. Harrell Malini Menon Rachel Brough James Campbell Jessica Frankum Michael Ranes Helen N. Pemberton Rumana Rafiq Kerry Fenwick Amanda Swain Sebastian Guettler Jung-Min Lee Elizabeth M. Swisher Stoyno Stoynov Kosuke Yusa Alan Ashworth Christopher J. Lord |
| author_facet |
Stephen J. Pettitt Dragomir B. Krastev Inger Brandsma Amy Dréan Feifei Song Radoslav Aleksandrov Maria I. Harrell Malini Menon Rachel Brough James Campbell Jessica Frankum Michael Ranes Helen N. Pemberton Rumana Rafiq Kerry Fenwick Amanda Swain Sebastian Guettler Jung-Min Lee Elizabeth M. Swisher Stoyno Stoynov Kosuke Yusa Alan Ashworth Christopher J. Lord |
| author_sort |
Stephen J. Pettitt |
| title |
Genome-wide and high-density CRISPR-Cas9 screens identify point mutations in PARP1 causing PARP inhibitor resistance |
| title_short |
Genome-wide and high-density CRISPR-Cas9 screens identify point mutations in PARP1 causing PARP inhibitor resistance |
| title_full |
Genome-wide and high-density CRISPR-Cas9 screens identify point mutations in PARP1 causing PARP inhibitor resistance |
| title_fullStr |
Genome-wide and high-density CRISPR-Cas9 screens identify point mutations in PARP1 causing PARP inhibitor resistance |
| title_full_unstemmed |
Genome-wide and high-density CRISPR-Cas9 screens identify point mutations in PARP1 causing PARP inhibitor resistance |
| title_sort |
genome-wide and high-density crispr-cas9 screens identify point mutations in parp1 causing parp inhibitor resistance |
| publisher |
Nature Portfolio |
| publishDate |
2018 |
| url |
https://doaj.org/article/1eb977c74fb546b0a7321e49be5d0643 |
| work_keys_str_mv |
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