Chronic cannabis smoking-enriched oral pathobiont drives behavioral changes, macrophage infiltration, and increases β-amyloid protein production in the brain
Background: Little is known about chronic cannabis smoking-associated oral microbiome and its effects on central nervous system (CNS) functions. Methods: In the current study, we have analyzed the saliva microbiome in individuals who chronically smoked cannabis with cannabis use disorder (n = 16) an...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Elsevier
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/1efa300385834349ae3f982625ed84e1 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:1efa300385834349ae3f982625ed84e1 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:1efa300385834349ae3f982625ed84e12021-11-24T04:31:57ZChronic cannabis smoking-enriched oral pathobiont drives behavioral changes, macrophage infiltration, and increases β-amyloid protein production in the brain2352-396410.1016/j.ebiom.2021.103701https://doaj.org/article/1efa300385834349ae3f982625ed84e12021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2352396421004953https://doaj.org/toc/2352-3964Background: Little is known about chronic cannabis smoking-associated oral microbiome and its effects on central nervous system (CNS) functions. Methods: In the current study, we have analyzed the saliva microbiome in individuals who chronically smoked cannabis with cannabis use disorder (n = 16) and in non-smoking controls (n = 27). The saliva microbiome was analyzed using microbial 16S rRNA sequencing. To investigate the function of cannabis use-associated oral microbiome, mice were orally inoculated with live Actinomyces meyeri, Actinomyces odontolyticus, or Neisseria elongata twice per week for six months, which mimicked human conditions. Findings: We found that cannabis smoking in humans was associated with oral microbial dysbiosis. The most increased oral bacteria were Streptococcus and Actinomyces genus and the most decreased bacteria were Neisseria genus in chronic cannabis smokers compared to those in non-smokers. Among the distinct species bacteria in cannabis smokers, the enrichment of Actinomyces meyeri was inversely associated with the age of first cannabis smoking. Strikingly, oral exposure of Actinomyces meyeri, an oral pathobiont, but not the other two control bacteria, decreased global activity, increased macrophage infiltration, and increased β-amyloid 42 protein production in the mouse brains. Interpretation: This is the first study to reveal that long-term oral cannabis exposure is associated oral enrichment of Actinomyces meyeri and its contributions to CNS abnormalities.Zhenwu LuoSylvia FittingCatrina RobinsonAndreana BenitezMin LiYongxia WuXiaoyu FuDavide AmatoWangbin NingNicholas FunderburgXu WangZejun ZhouXuezhong YuAmanda WagnerXiaomei CongWanli XuKendra MaasBethany J. WolfLei HuangJeremy YuAlison ScottAimee Mcrae-ClarkEric D. HamlettWei JiangElsevierarticleCannabis smokingOral microbiomeActinomyces meyeriβ-amyloidMedicineRMedicine (General)R5-920ENEBioMedicine, Vol 74, Iss , Pp 103701- (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Cannabis smoking Oral microbiome Actinomyces meyeri β-amyloid Medicine R Medicine (General) R5-920 |
| spellingShingle |
Cannabis smoking Oral microbiome Actinomyces meyeri β-amyloid Medicine R Medicine (General) R5-920 Zhenwu Luo Sylvia Fitting Catrina Robinson Andreana Benitez Min Li Yongxia Wu Xiaoyu Fu Davide Amato Wangbin Ning Nicholas Funderburg Xu Wang Zejun Zhou Xuezhong Yu Amanda Wagner Xiaomei Cong Wanli Xu Kendra Maas Bethany J. Wolf Lei Huang Jeremy Yu Alison Scott Aimee Mcrae-Clark Eric D. Hamlett Wei Jiang Chronic cannabis smoking-enriched oral pathobiont drives behavioral changes, macrophage infiltration, and increases β-amyloid protein production in the brain |
| description |
Background: Little is known about chronic cannabis smoking-associated oral microbiome and its effects on central nervous system (CNS) functions. Methods: In the current study, we have analyzed the saliva microbiome in individuals who chronically smoked cannabis with cannabis use disorder (n = 16) and in non-smoking controls (n = 27). The saliva microbiome was analyzed using microbial 16S rRNA sequencing. To investigate the function of cannabis use-associated oral microbiome, mice were orally inoculated with live Actinomyces meyeri, Actinomyces odontolyticus, or Neisseria elongata twice per week for six months, which mimicked human conditions. Findings: We found that cannabis smoking in humans was associated with oral microbial dysbiosis. The most increased oral bacteria were Streptococcus and Actinomyces genus and the most decreased bacteria were Neisseria genus in chronic cannabis smokers compared to those in non-smokers. Among the distinct species bacteria in cannabis smokers, the enrichment of Actinomyces meyeri was inversely associated with the age of first cannabis smoking. Strikingly, oral exposure of Actinomyces meyeri, an oral pathobiont, but not the other two control bacteria, decreased global activity, increased macrophage infiltration, and increased β-amyloid 42 protein production in the mouse brains. Interpretation: This is the first study to reveal that long-term oral cannabis exposure is associated oral enrichment of Actinomyces meyeri and its contributions to CNS abnormalities. |
| format |
article |
| author |
Zhenwu Luo Sylvia Fitting Catrina Robinson Andreana Benitez Min Li Yongxia Wu Xiaoyu Fu Davide Amato Wangbin Ning Nicholas Funderburg Xu Wang Zejun Zhou Xuezhong Yu Amanda Wagner Xiaomei Cong Wanli Xu Kendra Maas Bethany J. Wolf Lei Huang Jeremy Yu Alison Scott Aimee Mcrae-Clark Eric D. Hamlett Wei Jiang |
| author_facet |
Zhenwu Luo Sylvia Fitting Catrina Robinson Andreana Benitez Min Li Yongxia Wu Xiaoyu Fu Davide Amato Wangbin Ning Nicholas Funderburg Xu Wang Zejun Zhou Xuezhong Yu Amanda Wagner Xiaomei Cong Wanli Xu Kendra Maas Bethany J. Wolf Lei Huang Jeremy Yu Alison Scott Aimee Mcrae-Clark Eric D. Hamlett Wei Jiang |
| author_sort |
Zhenwu Luo |
| title |
Chronic cannabis smoking-enriched oral pathobiont drives behavioral changes, macrophage infiltration, and increases β-amyloid protein production in the brain |
| title_short |
Chronic cannabis smoking-enriched oral pathobiont drives behavioral changes, macrophage infiltration, and increases β-amyloid protein production in the brain |
| title_full |
Chronic cannabis smoking-enriched oral pathobiont drives behavioral changes, macrophage infiltration, and increases β-amyloid protein production in the brain |
| title_fullStr |
Chronic cannabis smoking-enriched oral pathobiont drives behavioral changes, macrophage infiltration, and increases β-amyloid protein production in the brain |
| title_full_unstemmed |
Chronic cannabis smoking-enriched oral pathobiont drives behavioral changes, macrophage infiltration, and increases β-amyloid protein production in the brain |
| title_sort |
chronic cannabis smoking-enriched oral pathobiont drives behavioral changes, macrophage infiltration, and increases β-amyloid protein production in the brain |
| publisher |
Elsevier |
| publishDate |
2021 |
| url |
https://doaj.org/article/1efa300385834349ae3f982625ed84e1 |
| work_keys_str_mv |
AT zhenwuluo chroniccannabissmokingenrichedoralpathobiontdrivesbehavioralchangesmacrophageinfiltrationandincreasesbamyloidproteinproductioninthebrain AT sylviafitting chroniccannabissmokingenrichedoralpathobiontdrivesbehavioralchangesmacrophageinfiltrationandincreasesbamyloidproteinproductioninthebrain AT catrinarobinson chroniccannabissmokingenrichedoralpathobiontdrivesbehavioralchangesmacrophageinfiltrationandincreasesbamyloidproteinproductioninthebrain AT andreanabenitez chroniccannabissmokingenrichedoralpathobiontdrivesbehavioralchangesmacrophageinfiltrationandincreasesbamyloidproteinproductioninthebrain AT minli chroniccannabissmokingenrichedoralpathobiontdrivesbehavioralchangesmacrophageinfiltrationandincreasesbamyloidproteinproductioninthebrain AT yongxiawu chroniccannabissmokingenrichedoralpathobiontdrivesbehavioralchangesmacrophageinfiltrationandincreasesbamyloidproteinproductioninthebrain AT xiaoyufu chroniccannabissmokingenrichedoralpathobiontdrivesbehavioralchangesmacrophageinfiltrationandincreasesbamyloidproteinproductioninthebrain AT davideamato chroniccannabissmokingenrichedoralpathobiontdrivesbehavioralchangesmacrophageinfiltrationandincreasesbamyloidproteinproductioninthebrain AT wangbinning chroniccannabissmokingenrichedoralpathobiontdrivesbehavioralchangesmacrophageinfiltrationandincreasesbamyloidproteinproductioninthebrain AT nicholasfunderburg chroniccannabissmokingenrichedoralpathobiontdrivesbehavioralchangesmacrophageinfiltrationandincreasesbamyloidproteinproductioninthebrain AT xuwang chroniccannabissmokingenrichedoralpathobiontdrivesbehavioralchangesmacrophageinfiltrationandincreasesbamyloidproteinproductioninthebrain AT zejunzhou chroniccannabissmokingenrichedoralpathobiontdrivesbehavioralchangesmacrophageinfiltrationandincreasesbamyloidproteinproductioninthebrain AT xuezhongyu chroniccannabissmokingenrichedoralpathobiontdrivesbehavioralchangesmacrophageinfiltrationandincreasesbamyloidproteinproductioninthebrain AT amandawagner chroniccannabissmokingenrichedoralpathobiontdrivesbehavioralchangesmacrophageinfiltrationandincreasesbamyloidproteinproductioninthebrain AT xiaomeicong chroniccannabissmokingenrichedoralpathobiontdrivesbehavioralchangesmacrophageinfiltrationandincreasesbamyloidproteinproductioninthebrain AT wanlixu chroniccannabissmokingenrichedoralpathobiontdrivesbehavioralchangesmacrophageinfiltrationandincreasesbamyloidproteinproductioninthebrain AT kendramaas chroniccannabissmokingenrichedoralpathobiontdrivesbehavioralchangesmacrophageinfiltrationandincreasesbamyloidproteinproductioninthebrain AT bethanyjwolf chroniccannabissmokingenrichedoralpathobiontdrivesbehavioralchangesmacrophageinfiltrationandincreasesbamyloidproteinproductioninthebrain AT leihuang chroniccannabissmokingenrichedoralpathobiontdrivesbehavioralchangesmacrophageinfiltrationandincreasesbamyloidproteinproductioninthebrain AT jeremyyu chroniccannabissmokingenrichedoralpathobiontdrivesbehavioralchangesmacrophageinfiltrationandincreasesbamyloidproteinproductioninthebrain AT alisonscott chroniccannabissmokingenrichedoralpathobiontdrivesbehavioralchangesmacrophageinfiltrationandincreasesbamyloidproteinproductioninthebrain AT aimeemcraeclark chroniccannabissmokingenrichedoralpathobiontdrivesbehavioralchangesmacrophageinfiltrationandincreasesbamyloidproteinproductioninthebrain AT ericdhamlett chroniccannabissmokingenrichedoralpathobiontdrivesbehavioralchangesmacrophageinfiltrationandincreasesbamyloidproteinproductioninthebrain AT weijiang chroniccannabissmokingenrichedoralpathobiontdrivesbehavioralchangesmacrophageinfiltrationandincreasesbamyloidproteinproductioninthebrain |
| _version_ |
1718415963120992256 |