DNA Backbone Sulfur-Modification Expands Microbial Growth Range under Multiple Stresses by its anti-oxidation function

Abstract DNA phosphorothioate (PT) modification is a sulfur modification on the backbone of DNA introduced by the proteins DndA-E. It has been detected within many bacteria isolates and metagenomic datasets, including human pathogens, and is considered to be widely distributed in nature. However, li...

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Autores principales:	Yan Yang, Guanpeng Xu, Jingdan Liang, Ying He, Lei Xiong, Hui Li, Douglas Bartlett, Zixin Deng, Zhijun Wang, Xiang Xiao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1ff47aaf03a04b9eb534c3de6f7b364a
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spelling	oai:doaj.org-article:1ff47aaf03a04b9eb534c3de6f7b364a2021-12-02T12:32:28ZDNA Backbone Sulfur-Modification Expands Microbial Growth Range under Multiple Stresses by its anti-oxidation function10.1038/s41598-017-02445-12045-2322https://doaj.org/article/1ff47aaf03a04b9eb534c3de6f7b364a2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02445-1https://doaj.org/toc/2045-2322Abstract DNA phosphorothioate (PT) modification is a sulfur modification on the backbone of DNA introduced by the proteins DndA-E. It has been detected within many bacteria isolates and metagenomic datasets, including human pathogens, and is considered to be widely distributed in nature. However, little is known about the physiological function of this modification, and thus its evolutionary significance and application potential remains largely a mystery. In this study, we focused on the advantages of DNA PT modification to bacterial cells coping with environmental stresses. We show that the mesophile Escherichia coli and the extremophile Shewanella piezotolerans both expanded their growth ranges following exposure to extreme temperature, salinity, pH, pressure, UV, X-ray and heavy metals as a result of DNA phophorothioation. The phophorothioated DNA reacted to both H2O2 and hydroxyl radicals in vivo, and protected genomic DNA as well as sensitive enzymes from intracellular oxidative damage. We further demonstrate that this process has evolved separate from its associated role in DNA restriction and modification. These findings provide a physiological role for a covalent modification widespread in nature and suggest possible applications in biotechnology and biomedicine.Yan YangGuanpeng XuJingdan LiangYing HeLei XiongHui LiDouglas BartlettZixin DengZhijun WangXiang XiaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yan Yang Guanpeng Xu Jingdan Liang Ying He Lei Xiong Hui Li Douglas Bartlett Zixin Deng Zhijun Wang Xiang Xiao DNA Backbone Sulfur-Modification Expands Microbial Growth Range under Multiple Stresses by its anti-oxidation function
description	Abstract DNA phosphorothioate (PT) modification is a sulfur modification on the backbone of DNA introduced by the proteins DndA-E. It has been detected within many bacteria isolates and metagenomic datasets, including human pathogens, and is considered to be widely distributed in nature. However, little is known about the physiological function of this modification, and thus its evolutionary significance and application potential remains largely a mystery. In this study, we focused on the advantages of DNA PT modification to bacterial cells coping with environmental stresses. We show that the mesophile Escherichia coli and the extremophile Shewanella piezotolerans both expanded their growth ranges following exposure to extreme temperature, salinity, pH, pressure, UV, X-ray and heavy metals as a result of DNA phophorothioation. The phophorothioated DNA reacted to both H2O2 and hydroxyl radicals in vivo, and protected genomic DNA as well as sensitive enzymes from intracellular oxidative damage. We further demonstrate that this process has evolved separate from its associated role in DNA restriction and modification. These findings provide a physiological role for a covalent modification widespread in nature and suggest possible applications in biotechnology and biomedicine.
format	article
author	Yan Yang Guanpeng Xu Jingdan Liang Ying He Lei Xiong Hui Li Douglas Bartlett Zixin Deng Zhijun Wang Xiang Xiao
author_facet	Yan Yang Guanpeng Xu Jingdan Liang Ying He Lei Xiong Hui Li Douglas Bartlett Zixin Deng Zhijun Wang Xiang Xiao
author_sort	Yan Yang
title	DNA Backbone Sulfur-Modification Expands Microbial Growth Range under Multiple Stresses by its anti-oxidation function
title_short	DNA Backbone Sulfur-Modification Expands Microbial Growth Range under Multiple Stresses by its anti-oxidation function
title_full	DNA Backbone Sulfur-Modification Expands Microbial Growth Range under Multiple Stresses by its anti-oxidation function
title_fullStr	DNA Backbone Sulfur-Modification Expands Microbial Growth Range under Multiple Stresses by its anti-oxidation function
title_full_unstemmed	DNA Backbone Sulfur-Modification Expands Microbial Growth Range under Multiple Stresses by its anti-oxidation function
title_sort	dna backbone sulfur-modification expands microbial growth range under multiple stresses by its anti-oxidation function
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1ff47aaf03a04b9eb534c3de6f7b364a
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DNA Backbone Sulfur-Modification Expands Microbial Growth Range under Multiple Stresses by its anti-oxidation function

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