Biglycan expression and its function in human ligamentum flavum

Abstract Hypertrophy of the ligamentum flavum (LF) is a major cause of lumbar spinal stenosis (LSS), and the pathology involves disruption of elastic fibers, fibrosis with increased cellularity and collagens, and/or calcification. Previous studies have implicated the increased expression of the prot...

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Autores principales: Hamidullah Salimi, Akinobu Suzuki, Hasibullah Habibi, Kumi Orita, Yusuke Hori, Akito Yabu, Hidetomi Terai, Koji Tamai, Hiroaki Nakamura
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Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/209eec5488334502a1ce1d2134a6521d
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spelling oai:doaj.org-article:209eec5488334502a1ce1d2134a6521d2021-12-02T13:35:05ZBiglycan expression and its function in human ligamentum flavum10.1038/s41598-021-84363-x2045-2322https://doaj.org/article/209eec5488334502a1ce1d2134a6521d2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84363-xhttps://doaj.org/toc/2045-2322Abstract Hypertrophy of the ligamentum flavum (LF) is a major cause of lumbar spinal stenosis (LSS), and the pathology involves disruption of elastic fibers, fibrosis with increased cellularity and collagens, and/or calcification. Previous studies have implicated the increased expression of the proteoglycan family in hypertrophied LF. Furthermore, the gene expression profile in a rabbit experimental model of LF hypertrophy revealed that biglycan (BGN) is upregulated in hypertrophied LF by mechanical stress. However, the expression and function of BGN in human LF has not been well elucidated. To investigate the involvement of BGN in the pathomechanism of human ligamentum hypertrophy, first we confirmed increased expression of BGN by immunohistochemistry in the extracellular matrix of hypertrophied LF of LSS patients compared to LF without hypertrophy. Experiments using primary cell cultures revealed that BGN promoted cell proliferation. Furthermore, BGN induces changes in cell morphology and promotes myofibroblastic differentiation and cell migration. These effects are observed for both cells from hypertrophied and non-hypertrophied LF. The present study revealed hyper-expression of BGN in hypertrophied LF and function of increased proteoglycan in LF cells. BGN may play a crucial role in the pathophysiology of LF hypertrophy through cell proliferation, myofibroblastic differentiation, and cell migration.Hamidullah SalimiAkinobu SuzukiHasibullah HabibiKumi OritaYusuke HoriAkito YabuHidetomi TeraiKoji TamaiHiroaki NakamuraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Hamidullah Salimi
Akinobu Suzuki
Hasibullah Habibi
Kumi Orita
Yusuke Hori
Akito Yabu
Hidetomi Terai
Koji Tamai
Hiroaki Nakamura
Biglycan expression and its function in human ligamentum flavum
description Abstract Hypertrophy of the ligamentum flavum (LF) is a major cause of lumbar spinal stenosis (LSS), and the pathology involves disruption of elastic fibers, fibrosis with increased cellularity and collagens, and/or calcification. Previous studies have implicated the increased expression of the proteoglycan family in hypertrophied LF. Furthermore, the gene expression profile in a rabbit experimental model of LF hypertrophy revealed that biglycan (BGN) is upregulated in hypertrophied LF by mechanical stress. However, the expression and function of BGN in human LF has not been well elucidated. To investigate the involvement of BGN in the pathomechanism of human ligamentum hypertrophy, first we confirmed increased expression of BGN by immunohistochemistry in the extracellular matrix of hypertrophied LF of LSS patients compared to LF without hypertrophy. Experiments using primary cell cultures revealed that BGN promoted cell proliferation. Furthermore, BGN induces changes in cell morphology and promotes myofibroblastic differentiation and cell migration. These effects are observed for both cells from hypertrophied and non-hypertrophied LF. The present study revealed hyper-expression of BGN in hypertrophied LF and function of increased proteoglycan in LF cells. BGN may play a crucial role in the pathophysiology of LF hypertrophy through cell proliferation, myofibroblastic differentiation, and cell migration.
format article
author Hamidullah Salimi
Akinobu Suzuki
Hasibullah Habibi
Kumi Orita
Yusuke Hori
Akito Yabu
Hidetomi Terai
Koji Tamai
Hiroaki Nakamura
author_facet Hamidullah Salimi
Akinobu Suzuki
Hasibullah Habibi
Kumi Orita
Yusuke Hori
Akito Yabu
Hidetomi Terai
Koji Tamai
Hiroaki Nakamura
author_sort Hamidullah Salimi
title Biglycan expression and its function in human ligamentum flavum
title_short Biglycan expression and its function in human ligamentum flavum
title_full Biglycan expression and its function in human ligamentum flavum
title_fullStr Biglycan expression and its function in human ligamentum flavum
title_full_unstemmed Biglycan expression and its function in human ligamentum flavum
title_sort biglycan expression and its function in human ligamentum flavum
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/209eec5488334502a1ce1d2134a6521d
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