Pulmonary toxicity and adjuvant effect of di-(2-exylhexyl) phthalate in ovalbumin-immunized BALB/c mice.
<h4>Background</h4>Asthma is a complex pulmonary inflammatory disease, which is characterized by airway hyperresponsiveness, variable airflow obstruction and inflammation in the airways. The majority of asthma is allergic asthma, which is a disease caused by type I hypersensitivity media...
Guardado en:
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2012
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/210eec0ff9f2449186eba5c478a7735a |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:210eec0ff9f2449186eba5c478a7735a |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:210eec0ff9f2449186eba5c478a7735a2021-11-18T07:15:44ZPulmonary toxicity and adjuvant effect of di-(2-exylhexyl) phthalate in ovalbumin-immunized BALB/c mice.1932-620310.1371/journal.pone.0039008https://doaj.org/article/210eec0ff9f2449186eba5c478a7735a2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22701742/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Asthma is a complex pulmonary inflammatory disease, which is characterized by airway hyperresponsiveness, variable airflow obstruction and inflammation in the airways. The majority of asthma is allergic asthma, which is a disease caused by type I hypersensitivity mediated by IgE. Exposures to a number of environmental chemicals are suspected to lead to asthma, one such pollutant is di-(2-ethylheyl) phthalate (DEHP). DEHP is a manufactured chemical that is commonly added in plastic products to make them flexible. Epidemiological studies have revealed a positive association between DEHP exposure and asthma prevalence.<h4>Methodology/principal findings</h4>The present study was aimed to determine the underlying role of DEHP exposure in airway reactivity, especially when combined with allergen exposure. The biomarkers include pulmonary histopathology, airway hyperresponsiveness (lung function), IgE, IL-4, IFN-γ and eosinophils. Healthy balb/c mice were randomly divided into eight exposure groups (n = 8 each): (1) saline control, (2) 30 µg/(kg•d) DEHP, (3) 300 µg/(kg•d) DEHP, (4) 3000 µg/(kg•d) DEHP, and (5) ovalbumin (OVA)-sensitized group, (6) OVA-combined with 30 µg/(kg•d) DEHP, (7) OVA-combined with 300 µg/(kg•d) DEHP, and (8) OVA-combined with 3000 µg/(kg•d) DEHP. Experimental tests were conducted after 52-day DEHP exposure and subsequently one week of challenge with aerosolized OVA. The principal findings include: (1) Strong postive associations exist between OVA-combined DEHP exposure and serum total IgE (T-IgE), as well as histological findings. These positive associations show a dose-dependent low dose sensitive effect of DEHP. (2) IL-4, eosinophil recruitment and lung function are also indicators for adjuvant effect of DEHP.<h4>Conclusions/significance</h4>Our results suggest that except the significant changes of immunological and inflammatory biomarkers (T-IgE, IL-4, IFN-γ and eosinophils), the pulmonary histological (histopathological examination) and physiological (lung function) data also support that DEHP may promote and aggravate allergic asthma by adjuvant effect.Jing GuoBing HanLongjuan QinBing LiHuihui YouJiwen YangDandan LiuChenxi WeiEewa NanbergCarl-Gustaf BornehagXu YangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 6, p e39008 (2012) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Jing Guo Bing Han Longjuan Qin Bing Li Huihui You Jiwen Yang Dandan Liu Chenxi Wei Eewa Nanberg Carl-Gustaf Bornehag Xu Yang Pulmonary toxicity and adjuvant effect of di-(2-exylhexyl) phthalate in ovalbumin-immunized BALB/c mice. |
| description |
<h4>Background</h4>Asthma is a complex pulmonary inflammatory disease, which is characterized by airway hyperresponsiveness, variable airflow obstruction and inflammation in the airways. The majority of asthma is allergic asthma, which is a disease caused by type I hypersensitivity mediated by IgE. Exposures to a number of environmental chemicals are suspected to lead to asthma, one such pollutant is di-(2-ethylheyl) phthalate (DEHP). DEHP is a manufactured chemical that is commonly added in plastic products to make them flexible. Epidemiological studies have revealed a positive association between DEHP exposure and asthma prevalence.<h4>Methodology/principal findings</h4>The present study was aimed to determine the underlying role of DEHP exposure in airway reactivity, especially when combined with allergen exposure. The biomarkers include pulmonary histopathology, airway hyperresponsiveness (lung function), IgE, IL-4, IFN-γ and eosinophils. Healthy balb/c mice were randomly divided into eight exposure groups (n = 8 each): (1) saline control, (2) 30 µg/(kg•d) DEHP, (3) 300 µg/(kg•d) DEHP, (4) 3000 µg/(kg•d) DEHP, and (5) ovalbumin (OVA)-sensitized group, (6) OVA-combined with 30 µg/(kg•d) DEHP, (7) OVA-combined with 300 µg/(kg•d) DEHP, and (8) OVA-combined with 3000 µg/(kg•d) DEHP. Experimental tests were conducted after 52-day DEHP exposure and subsequently one week of challenge with aerosolized OVA. The principal findings include: (1) Strong postive associations exist between OVA-combined DEHP exposure and serum total IgE (T-IgE), as well as histological findings. These positive associations show a dose-dependent low dose sensitive effect of DEHP. (2) IL-4, eosinophil recruitment and lung function are also indicators for adjuvant effect of DEHP.<h4>Conclusions/significance</h4>Our results suggest that except the significant changes of immunological and inflammatory biomarkers (T-IgE, IL-4, IFN-γ and eosinophils), the pulmonary histological (histopathological examination) and physiological (lung function) data also support that DEHP may promote and aggravate allergic asthma by adjuvant effect. |
| format |
article |
| author |
Jing Guo Bing Han Longjuan Qin Bing Li Huihui You Jiwen Yang Dandan Liu Chenxi Wei Eewa Nanberg Carl-Gustaf Bornehag Xu Yang |
| author_facet |
Jing Guo Bing Han Longjuan Qin Bing Li Huihui You Jiwen Yang Dandan Liu Chenxi Wei Eewa Nanberg Carl-Gustaf Bornehag Xu Yang |
| author_sort |
Jing Guo |
| title |
Pulmonary toxicity and adjuvant effect of di-(2-exylhexyl) phthalate in ovalbumin-immunized BALB/c mice. |
| title_short |
Pulmonary toxicity and adjuvant effect of di-(2-exylhexyl) phthalate in ovalbumin-immunized BALB/c mice. |
| title_full |
Pulmonary toxicity and adjuvant effect of di-(2-exylhexyl) phthalate in ovalbumin-immunized BALB/c mice. |
| title_fullStr |
Pulmonary toxicity and adjuvant effect of di-(2-exylhexyl) phthalate in ovalbumin-immunized BALB/c mice. |
| title_full_unstemmed |
Pulmonary toxicity and adjuvant effect of di-(2-exylhexyl) phthalate in ovalbumin-immunized BALB/c mice. |
| title_sort |
pulmonary toxicity and adjuvant effect of di-(2-exylhexyl) phthalate in ovalbumin-immunized balb/c mice. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/210eec0ff9f2449186eba5c478a7735a |
| work_keys_str_mv |
AT jingguo pulmonarytoxicityandadjuvanteffectofdi2exylhexylphthalateinovalbuminimmunizedbalbcmice AT binghan pulmonarytoxicityandadjuvanteffectofdi2exylhexylphthalateinovalbuminimmunizedbalbcmice AT longjuanqin pulmonarytoxicityandadjuvanteffectofdi2exylhexylphthalateinovalbuminimmunizedbalbcmice AT bingli pulmonarytoxicityandadjuvanteffectofdi2exylhexylphthalateinovalbuminimmunizedbalbcmice AT huihuiyou pulmonarytoxicityandadjuvanteffectofdi2exylhexylphthalateinovalbuminimmunizedbalbcmice AT jiwenyang pulmonarytoxicityandadjuvanteffectofdi2exylhexylphthalateinovalbuminimmunizedbalbcmice AT dandanliu pulmonarytoxicityandadjuvanteffectofdi2exylhexylphthalateinovalbuminimmunizedbalbcmice AT chenxiwei pulmonarytoxicityandadjuvanteffectofdi2exylhexylphthalateinovalbuminimmunizedbalbcmice AT eewananberg pulmonarytoxicityandadjuvanteffectofdi2exylhexylphthalateinovalbuminimmunizedbalbcmice AT carlgustafbornehag pulmonarytoxicityandadjuvanteffectofdi2exylhexylphthalateinovalbuminimmunizedbalbcmice AT xuyang pulmonarytoxicityandadjuvanteffectofdi2exylhexylphthalateinovalbuminimmunizedbalbcmice |
| _version_ |
1718423713032962048 |