Optimized protocol for the extraction of RNA and DNA from frozen whole blood sample stored in a single EDTA tube

Optimized protocol for the extraction of RNA and DNA from frozen whole blood sample stored in a single EDTA tube

Abstract Cryopreservation of whole blood is useful for DNA collection, and clinical and basic research. Blood samples in ethylenediaminetetraacetic acid disodium salt (EDTA) tubes stored at − 80 °C are suitable for DNA extraction, but not for high-quality RNA extraction. Herein, a new methodology fo...

Description complète

Enregistré dans:
Détails bibliographiques
Auteurs principaux: Hirotaka Yamagata, Ayumi Kobayashi, Ryouichi Tsunedomi, Tomoe Seki, Masaaki Kobayashi, Kosuke Hagiwara, Chong Chen, Shusaku Uchida, Go Okada, Manabu Fuchikami, Toshiharu Kamishikiryo, Jun-ichi Iga, Shusuke Numata, Makoto Kinoshita, Takahiro A. Kato, Ryota Hashimoto, Hiroaki Nagano, Yasumasa Okamoto, Shuichi Ueno, Tetsuro Ohmori, Shin Nakagawa
Format: article
Langue:EN
Publié: Nature Portfolio 2021
Sujets:
Medicine
R
Science
Q
Accès en ligne:https://doaj.org/article/212124c659ab4ed4bcf4e9068db01cdf
Tags: Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!

Internet

https://doaj.org/article/212124c659ab4ed4bcf4e9068db01cdf

Documents similaires