An in situ activity assay for lysyl oxidases

An in situ activity assay for lysyl oxidases

To address the limitation of inability to detect lysyl oxidase enzymes (LOX) catalytic function associated with diverse disease processes, Wang et al. developed an in situ activity assay by utilizing the reactivity of hydrazides with imines and carbonyls. This assay detects the total LOX activity in...

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Autores principales: Huilei Wang, Alan Poe, Lydia Pak, Kavitha Nandakumar, Sandeep Jandu, Jochen Steppan, Reik Löser, Lakshmi Santhanam
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Biology (General)
QH301-705.5
Acceso en línea:https://doaj.org/article/226aeaa38a774c2ebbf2d0e380dfffce
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spelling oai:doaj.org-article:226aeaa38a774c2ebbf2d0e380dfffce2021-12-02T16:15:04ZAn in situ activity assay for lysyl oxidases10.1038/s42003-021-02354-02399-3642https://doaj.org/article/226aeaa38a774c2ebbf2d0e380dfffce2021-07-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02354-0https://doaj.org/toc/2399-3642To address the limitation of inability to detect lysyl oxidase enzymes (LOX) catalytic function associated with diverse disease processes, Wang et al. developed an in situ activity assay by utilizing the reactivity of hydrazides with imines and carbonyls. This assay detects the total LOX activity in situ for both overexpressed and endogenous LOXs in cells and tissue samples.Huilei WangAlan PoeLydia PakKavitha NandakumarSandeep JanduJochen SteppanReik LöserLakshmi SanthanamNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-10 (2021)
institution DOAJ
collection DOAJ
language EN
topic Biology (General)
QH301-705.5
spellingShingle Biology (General)
QH301-705.5
Huilei Wang
Alan Poe
Lydia Pak
Kavitha Nandakumar
Sandeep Jandu
Jochen Steppan
Reik Löser
Lakshmi Santhanam
An in situ activity assay for lysyl oxidases
description To address the limitation of inability to detect lysyl oxidase enzymes (LOX) catalytic function associated with diverse disease processes, Wang et al. developed an in situ activity assay by utilizing the reactivity of hydrazides with imines and carbonyls. This assay detects the total LOX activity in situ for both overexpressed and endogenous LOXs in cells and tissue samples.
format article
author Huilei Wang
Alan Poe
Lydia Pak
Kavitha Nandakumar
Sandeep Jandu
Jochen Steppan
Reik Löser
Lakshmi Santhanam
author_facet Huilei Wang
Alan Poe
Lydia Pak
Kavitha Nandakumar
Sandeep Jandu
Jochen Steppan
Reik Löser
Lakshmi Santhanam
author_sort Huilei Wang
title An in situ activity assay for lysyl oxidases
title_short An in situ activity assay for lysyl oxidases
title_full An in situ activity assay for lysyl oxidases
title_fullStr An in situ activity assay for lysyl oxidases
title_full_unstemmed An in situ activity assay for lysyl oxidases
title_sort in situ activity assay for lysyl oxidases
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/226aeaa38a774c2ebbf2d0e380dfffce
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