Examination of the Catalytic Role of the Axial Cystine Ligand in the Co-Type Nitrile Hydratase from <i>Pseudonocardia thermophila</i> JCM 3095

The strictly conserved αSer162 residue in the Co-type nitrile hydratase from <i>Pseudonocardia thermophila</i> JCM 3095 (<i>Pt</i>NHase), which forms a hydrogen bond to the axial αCys108-S atom, was mutated into an Ala residue. The αSer162Ala yielded two different protein spe...

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Auteurs principaux: Irene R. A. M. Ogutu, Martin St. Maurice, Brian Bennett, Richard C. Holz
Format: article
Langue:EN
Publié: MDPI AG 2021
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Accès en ligne:https://doaj.org/article/22a456b625b1437fa980c0190a0a18e0
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