A standardized genome architecture for bacterial synthetic biology (SEGA)

Genome engineering is challenging compared to plasmid DNA manipulation. Here the authors create a simple methodology called SEGA that enables genome engineering by combining DNA and bacterial cells followed by identification of recombinant clones by a change in colour when grown on agar plates.

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Autores principales: Carolyn N. Bayer, Maja Rennig, Anja K. Ehrmann, Morten H. H. Nørholm
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/22f6fac5cbeb4aea9cfc13c6aad002c5
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