A standardized genome architecture for bacterial synthetic biology (SEGA)

Genome engineering is challenging compared to plasmid DNA manipulation. Here the authors create a simple methodology called SEGA that enables genome engineering by combining DNA and bacterial cells followed by identification of recombinant clones by a change in colour when grown on agar plates.

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Autores principales:	Carolyn N. Bayer, Maja Rennig, Anja K. Ehrmann, Morten H. H. Nørholm
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/22f6fac5cbeb4aea9cfc13c6aad002c5
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spelling	oai:doaj.org-article:22f6fac5cbeb4aea9cfc13c6aad002c52021-12-02T18:08:47ZA standardized genome architecture for bacterial synthetic biology (SEGA)10.1038/s41467-021-26155-52041-1723https://doaj.org/article/22f6fac5cbeb4aea9cfc13c6aad002c52021-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-26155-5https://doaj.org/toc/2041-1723Genome engineering is challenging compared to plasmid DNA manipulation. Here the authors create a simple methodology called SEGA that enables genome engineering by combining DNA and bacterial cells followed by identification of recombinant clones by a change in colour when grown on agar plates.Carolyn N. BayerMaja RennigAnja K. EhrmannMorten H. H. NørholmNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Carolyn N. Bayer Maja Rennig Anja K. Ehrmann Morten H. H. Nørholm A standardized genome architecture for bacterial synthetic biology (SEGA)
description	Genome engineering is challenging compared to plasmid DNA manipulation. Here the authors create a simple methodology called SEGA that enables genome engineering by combining DNA and bacterial cells followed by identification of recombinant clones by a change in colour when grown on agar plates.
format	article
author	Carolyn N. Bayer Maja Rennig Anja K. Ehrmann Morten H. H. Nørholm
author_facet	Carolyn N. Bayer Maja Rennig Anja K. Ehrmann Morten H. H. Nørholm
author_sort	Carolyn N. Bayer
title	A standardized genome architecture for bacterial synthetic biology (SEGA)
title_short	A standardized genome architecture for bacterial synthetic biology (SEGA)
title_full	A standardized genome architecture for bacterial synthetic biology (SEGA)
title_fullStr	A standardized genome architecture for bacterial synthetic biology (SEGA)
title_full_unstemmed	A standardized genome architecture for bacterial synthetic biology (SEGA)
title_sort	standardized genome architecture for bacterial synthetic biology (sega)
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/22f6fac5cbeb4aea9cfc13c6aad002c5
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A standardized genome architecture for bacterial synthetic biology (SEGA)

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