A standardized genome architecture for bacterial synthetic biology (SEGA)
Genome engineering is challenging compared to plasmid DNA manipulation. Here the authors create a simple methodology called SEGA that enables genome engineering by combining DNA and bacterial cells followed by identification of recombinant clones by a change in colour when grown on agar plates.
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Main Authors: | Carolyn N. Bayer, Maja Rennig, Anja K. Ehrmann, Morten H. H. Nørholm |
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Format: | article |
Language: | EN |
Published: |
Nature Portfolio
2021
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Online Access: | https://doaj.org/article/22f6fac5cbeb4aea9cfc13c6aad002c5 |
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