Atomic Force Microscopy micro-rheology reveals large structural inhomogeneities in single cell-nuclei

Abstract During growth, differentiation and migration of cells, the nucleus changes size and shape, while encountering forces generated by the cell itself and its environment. Although there is increasing evidence that such mechanical signals are employed to control gene expression, it remains uncle...

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Autores principales:	Michael Lherbette, Ália dos Santos, Yukti Hari-Gupta, Natalia Fili, Christopher P. Toseland, Iwan A. T. Schaap
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/238f6000e7a14ac5ab9064a834e36ab6
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spelling	oai:doaj.org-article:238f6000e7a14ac5ab9064a834e36ab62021-12-02T11:40:59ZAtomic Force Microscopy micro-rheology reveals large structural inhomogeneities in single cell-nuclei10.1038/s41598-017-08517-62045-2322https://doaj.org/article/238f6000e7a14ac5ab9064a834e36ab62017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08517-6https://doaj.org/toc/2045-2322Abstract During growth, differentiation and migration of cells, the nucleus changes size and shape, while encountering forces generated by the cell itself and its environment. Although there is increasing evidence that such mechanical signals are employed to control gene expression, it remains unclear how mechanical forces are transduced through the nucleus. To this end, we have measured the compliance of nuclei by applying oscillatory strains between 1 and 700 Hz to individual nuclei of multiple mammalian cell-lines that were compressed between two plates. The quantitative response varied with more than one order of magnitude and scaled with the size of the nucleus. Surprisingly, the qualitative behaviour was conserved among different cell-lines: all nuclei showed a softer and more viscous response towards the periphery, suggesting a reduced degree of crosslinking of the chromatin. This may be an important feature to regulate transcription via mechano-transduction in this most active and dynamic region of the nucleus.Michael LherbetteÁlia dos SantosYukti Hari-GuptaNatalia FiliChristopher P. ToselandIwan A. T. SchaapNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michael Lherbette Ália dos Santos Yukti Hari-Gupta Natalia Fili Christopher P. Toseland Iwan A. T. Schaap Atomic Force Microscopy micro-rheology reveals large structural inhomogeneities in single cell-nuclei
description	Abstract During growth, differentiation and migration of cells, the nucleus changes size and shape, while encountering forces generated by the cell itself and its environment. Although there is increasing evidence that such mechanical signals are employed to control gene expression, it remains unclear how mechanical forces are transduced through the nucleus. To this end, we have measured the compliance of nuclei by applying oscillatory strains between 1 and 700 Hz to individual nuclei of multiple mammalian cell-lines that were compressed between two plates. The quantitative response varied with more than one order of magnitude and scaled with the size of the nucleus. Surprisingly, the qualitative behaviour was conserved among different cell-lines: all nuclei showed a softer and more viscous response towards the periphery, suggesting a reduced degree of crosslinking of the chromatin. This may be an important feature to regulate transcription via mechano-transduction in this most active and dynamic region of the nucleus.
format	article
author	Michael Lherbette Ália dos Santos Yukti Hari-Gupta Natalia Fili Christopher P. Toseland Iwan A. T. Schaap
author_facet	Michael Lherbette Ália dos Santos Yukti Hari-Gupta Natalia Fili Christopher P. Toseland Iwan A. T. Schaap
author_sort	Michael Lherbette
title	Atomic Force Microscopy micro-rheology reveals large structural inhomogeneities in single cell-nuclei
title_short	Atomic Force Microscopy micro-rheology reveals large structural inhomogeneities in single cell-nuclei
title_full	Atomic Force Microscopy micro-rheology reveals large structural inhomogeneities in single cell-nuclei
title_fullStr	Atomic Force Microscopy micro-rheology reveals large structural inhomogeneities in single cell-nuclei
title_full_unstemmed	Atomic Force Microscopy micro-rheology reveals large structural inhomogeneities in single cell-nuclei
title_sort	atomic force microscopy micro-rheology reveals large structural inhomogeneities in single cell-nuclei
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/238f6000e7a14ac5ab9064a834e36ab6
work_keys_str_mv	AT michaellherbette atomicforcemicroscopymicrorheologyrevealslargestructuralinhomogeneitiesinsinglecellnuclei AT aliadossantos atomicforcemicroscopymicrorheologyrevealslargestructuralinhomogeneitiesinsinglecellnuclei AT yuktiharigupta atomicforcemicroscopymicrorheologyrevealslargestructuralinhomogeneitiesinsinglecellnuclei AT nataliafili atomicforcemicroscopymicrorheologyrevealslargestructuralinhomogeneitiesinsinglecellnuclei AT christopherptoseland atomicforcemicroscopymicrorheologyrevealslargestructuralinhomogeneitiesinsinglecellnuclei AT iwanatschaap atomicforcemicroscopymicrorheologyrevealslargestructuralinhomogeneitiesinsinglecellnuclei
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Atomic Force Microscopy micro-rheology reveals large structural inhomogeneities in single cell-nuclei

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