PKM2 promotes cell migration and inhibits autophagy by mediating PI3K/AKT activation and contributes to the malignant development of gastric cancer

Abstract Pyruvate kinase M2 (PKM2) is a key kinase of glycolysis and is characteristic of all proliferating cells. The role of PKM2 in gastric cancer (GC) is still ambiguous and yet to be determined. To better understand the role of PKM2 in both the migration and invasion of GC, we measured the expr...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chao Wang, Jinling Jiang, Jun Ji, Qu Cai, Xuehua Chen, Yingyan Yu, Zhenggang Zhu, Jun Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/23d2295ff2db4daa815424bcfa67c565
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:23d2295ff2db4daa815424bcfa67c565
record_format	dspace
spelling	oai:doaj.org-article:23d2295ff2db4daa815424bcfa67c5652021-12-02T11:52:29ZPKM2 promotes cell migration and inhibits autophagy by mediating PI3K/AKT activation and contributes to the malignant development of gastric cancer10.1038/s41598-017-03031-12045-2322https://doaj.org/article/23d2295ff2db4daa815424bcfa67c5652017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03031-1https://doaj.org/toc/2045-2322Abstract Pyruvate kinase M2 (PKM2) is a key kinase of glycolysis and is characteristic of all proliferating cells. The role of PKM2 in gastric cancer (GC) is still ambiguous and yet to be determined. To better understand the role of PKM2 in both the migration and invasion of GC, we measured the expression of PKM2 in GC cell lines using qRT-PCR and western blot. The prognostic value of PKM2 was analyzed by Immunohistochemistry in a cohort containing 88 GC patients. PKM2 was knocked down by the short hairpin RNA plasmid vector in NCI-N87 and BGC-823 cells, and the biological behavior and downstream signaling pathways were also investigated in vitro. Subcutaneous xenografts and pulmonary metastases models were constructed in nude mice to compare the differences in tumorgenesis and metastasis after Knockdown of PKM2. Our results obtained from in vitro cell biological behavior, in vivo tumorigenicity studies, and primary GC samples revealed an oncogenic role for PKM2 in GC. Furthermore, for those GC patients who received radical resection, PKM2 might serve as a novel prognostic biomarker and target which would allow for a brand new treatment strategy for GC in the clinical settings.Chao WangJinling JiangJun JiQu CaiXuehua ChenYingyan YuZhenggang ZhuJun ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chao Wang Jinling Jiang Jun Ji Qu Cai Xuehua Chen Yingyan Yu Zhenggang Zhu Jun Zhang PKM2 promotes cell migration and inhibits autophagy by mediating PI3K/AKT activation and contributes to the malignant development of gastric cancer
description	Abstract Pyruvate kinase M2 (PKM2) is a key kinase of glycolysis and is characteristic of all proliferating cells. The role of PKM2 in gastric cancer (GC) is still ambiguous and yet to be determined. To better understand the role of PKM2 in both the migration and invasion of GC, we measured the expression of PKM2 in GC cell lines using qRT-PCR and western blot. The prognostic value of PKM2 was analyzed by Immunohistochemistry in a cohort containing 88 GC patients. PKM2 was knocked down by the short hairpin RNA plasmid vector in NCI-N87 and BGC-823 cells, and the biological behavior and downstream signaling pathways were also investigated in vitro. Subcutaneous xenografts and pulmonary metastases models were constructed in nude mice to compare the differences in tumorgenesis and metastasis after Knockdown of PKM2. Our results obtained from in vitro cell biological behavior, in vivo tumorigenicity studies, and primary GC samples revealed an oncogenic role for PKM2 in GC. Furthermore, for those GC patients who received radical resection, PKM2 might serve as a novel prognostic biomarker and target which would allow for a brand new treatment strategy for GC in the clinical settings.
format	article
author	Chao Wang Jinling Jiang Jun Ji Qu Cai Xuehua Chen Yingyan Yu Zhenggang Zhu Jun Zhang
author_facet	Chao Wang Jinling Jiang Jun Ji Qu Cai Xuehua Chen Yingyan Yu Zhenggang Zhu Jun Zhang
author_sort	Chao Wang
title	PKM2 promotes cell migration and inhibits autophagy by mediating PI3K/AKT activation and contributes to the malignant development of gastric cancer
title_short	PKM2 promotes cell migration and inhibits autophagy by mediating PI3K/AKT activation and contributes to the malignant development of gastric cancer
title_full	PKM2 promotes cell migration and inhibits autophagy by mediating PI3K/AKT activation and contributes to the malignant development of gastric cancer
title_fullStr	PKM2 promotes cell migration and inhibits autophagy by mediating PI3K/AKT activation and contributes to the malignant development of gastric cancer
title_full_unstemmed	PKM2 promotes cell migration and inhibits autophagy by mediating PI3K/AKT activation and contributes to the malignant development of gastric cancer
title_sort	pkm2 promotes cell migration and inhibits autophagy by mediating pi3k/akt activation and contributes to the malignant development of gastric cancer
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/23d2295ff2db4daa815424bcfa67c565
work_keys_str_mv	AT chaowang pkm2promotescellmigrationandinhibitsautophagybymediatingpi3kaktactivationandcontributestothemalignantdevelopmentofgastriccancer AT jinlingjiang pkm2promotescellmigrationandinhibitsautophagybymediatingpi3kaktactivationandcontributestothemalignantdevelopmentofgastriccancer AT junji pkm2promotescellmigrationandinhibitsautophagybymediatingpi3kaktactivationandcontributestothemalignantdevelopmentofgastriccancer AT qucai pkm2promotescellmigrationandinhibitsautophagybymediatingpi3kaktactivationandcontributestothemalignantdevelopmentofgastriccancer AT xuehuachen pkm2promotescellmigrationandinhibitsautophagybymediatingpi3kaktactivationandcontributestothemalignantdevelopmentofgastriccancer AT yingyanyu pkm2promotescellmigrationandinhibitsautophagybymediatingpi3kaktactivationandcontributestothemalignantdevelopmentofgastriccancer AT zhenggangzhu pkm2promotescellmigrationandinhibitsautophagybymediatingpi3kaktactivationandcontributestothemalignantdevelopmentofgastriccancer AT junzhang pkm2promotescellmigrationandinhibitsautophagybymediatingpi3kaktactivationandcontributestothemalignantdevelopmentofgastriccancer
_version_	1718395035089633280

PKM2 promotes cell migration and inhibits autophagy by mediating PI3K/AKT activation and contributes to the malignant development of gastric cancer

Ejemplares similares