Alternative activation of macrophages and induction of arginase are not components of pathogenesis mediated by Francisella species.

Virulent Francisella tularensis ssp tularensis is an intracellular, Gram negative bacterium that causes acute lethal disease following inhalation of fewer than 15 organisms. Pathogenicity of Francisella infections is tied to its unique ability to evade and suppress inflammatory responses in host cel...

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Autores principales:	Amanda J Griffin, Deborah D Crane, Tara D Wehrly, Dana P Scott, Catharine M Bosio
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/240a6df3c82d472697796ca7109b6c65
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spelling	oai:doaj.org-article:240a6df3c82d472697796ca7109b6c652021-11-18T08:43:08ZAlternative activation of macrophages and induction of arginase are not components of pathogenesis mediated by Francisella species.1932-620310.1371/journal.pone.0082096https://doaj.org/article/240a6df3c82d472697796ca7109b6c652013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24324751/?tool=EBIhttps://doaj.org/toc/1932-6203Virulent Francisella tularensis ssp tularensis is an intracellular, Gram negative bacterium that causes acute lethal disease following inhalation of fewer than 15 organisms. Pathogenicity of Francisella infections is tied to its unique ability to evade and suppress inflammatory responses in host cells. It has been proposed that induction of alternative activation of infected macrophages is a mechanism by which attenuated Francisella species modulate host responses. In this report we reveal that neither attenuated F. tularensis Live Vaccine Strain (LVS) nor virulent F. tularensis strain SchuS4 induce alternative activation of macrophages in vitro or in vivo. LVS, but not SchuS4, provoked production of arginase1 independent of alternative activation in vitro and in vivo. However, absence of arginase1 did not significantly impact intracellular replication of LVS or SchuS4. Together our data establish that neither induction of alternative activation nor expression of arginase1 are critical features of disease mediated by attenuated or virulent Francisella species.Amanda J GriffinDeborah D CraneTara D WehrlyDana P ScottCatharine M BosioPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e82096 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amanda J Griffin Deborah D Crane Tara D Wehrly Dana P Scott Catharine M Bosio Alternative activation of macrophages and induction of arginase are not components of pathogenesis mediated by Francisella species.
description	Virulent Francisella tularensis ssp tularensis is an intracellular, Gram negative bacterium that causes acute lethal disease following inhalation of fewer than 15 organisms. Pathogenicity of Francisella infections is tied to its unique ability to evade and suppress inflammatory responses in host cells. It has been proposed that induction of alternative activation of infected macrophages is a mechanism by which attenuated Francisella species modulate host responses. In this report we reveal that neither attenuated F. tularensis Live Vaccine Strain (LVS) nor virulent F. tularensis strain SchuS4 induce alternative activation of macrophages in vitro or in vivo. LVS, but not SchuS4, provoked production of arginase1 independent of alternative activation in vitro and in vivo. However, absence of arginase1 did not significantly impact intracellular replication of LVS or SchuS4. Together our data establish that neither induction of alternative activation nor expression of arginase1 are critical features of disease mediated by attenuated or virulent Francisella species.
format	article
author	Amanda J Griffin Deborah D Crane Tara D Wehrly Dana P Scott Catharine M Bosio
author_facet	Amanda J Griffin Deborah D Crane Tara D Wehrly Dana P Scott Catharine M Bosio
author_sort	Amanda J Griffin
title	Alternative activation of macrophages and induction of arginase are not components of pathogenesis mediated by Francisella species.
title_short	Alternative activation of macrophages and induction of arginase are not components of pathogenesis mediated by Francisella species.
title_full	Alternative activation of macrophages and induction of arginase are not components of pathogenesis mediated by Francisella species.
title_fullStr	Alternative activation of macrophages and induction of arginase are not components of pathogenesis mediated by Francisella species.
title_full_unstemmed	Alternative activation of macrophages and induction of arginase are not components of pathogenesis mediated by Francisella species.
title_sort	alternative activation of macrophages and induction of arginase are not components of pathogenesis mediated by francisella species.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/240a6df3c82d472697796ca7109b6c65
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_version_	1718421370689290240

Alternative activation of macrophages and induction of arginase are not components of pathogenesis mediated by Francisella species.

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