Cell-fate decision of mesenchymal stem cells toward osteocyte differentiation is committed by spheroid culture

Abstract Osteocytes are mechanosensory commander cells to regulate bone remodeling throughout the lifespan. While the osteocytes are known as terminally differentiated cells derived from mesenchymal stem cells, the detailed mechanisms of osteocyte differentiation remain unclear. In this study, we fa...

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Autores principales:	Jeonghyun Kim, Taiji Adachi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2425ffd5bce84a2d9252cadcd93ffc76
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spelling	oai:doaj.org-article:2425ffd5bce84a2d9252cadcd93ffc762021-12-02T17:12:25ZCell-fate decision of mesenchymal stem cells toward osteocyte differentiation is committed by spheroid culture10.1038/s41598-021-92607-z2045-2322https://doaj.org/article/2425ffd5bce84a2d9252cadcd93ffc762021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92607-zhttps://doaj.org/toc/2045-2322Abstract Osteocytes are mechanosensory commander cells to regulate bone remodeling throughout the lifespan. While the osteocytes are known as terminally differentiated cells derived from mesenchymal stem cells, the detailed mechanisms of osteocyte differentiation remain unclear. In this study, we fabricated 3D self-organized spheroids using human mesenchymal stem cells (MSCs). Under the osteogenesis induction medium, the spheroid culture model exerted the osteocyte-likeness within 2 days compared to a conventional 2D monolayer model. Moreover, we showed that an inhibition of actin polymerization in the spheroid further up-regulated the osteocyte gene expressions. Notably, we represented that the cell condensed condition acquired in the 3D spheroid culture model determined a differentiation fate of MSCs to osteocytes. Taken together, we suggest that our self-organized spheroid model can be utilized as a new in vitro model to represent the osteocyte and to recapitulate an in vitro ossification process.Jeonghyun KimTaiji AdachiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jeonghyun Kim Taiji Adachi Cell-fate decision of mesenchymal stem cells toward osteocyte differentiation is committed by spheroid culture
description	Abstract Osteocytes are mechanosensory commander cells to regulate bone remodeling throughout the lifespan. While the osteocytes are known as terminally differentiated cells derived from mesenchymal stem cells, the detailed mechanisms of osteocyte differentiation remain unclear. In this study, we fabricated 3D self-organized spheroids using human mesenchymal stem cells (MSCs). Under the osteogenesis induction medium, the spheroid culture model exerted the osteocyte-likeness within 2 days compared to a conventional 2D monolayer model. Moreover, we showed that an inhibition of actin polymerization in the spheroid further up-regulated the osteocyte gene expressions. Notably, we represented that the cell condensed condition acquired in the 3D spheroid culture model determined a differentiation fate of MSCs to osteocytes. Taken together, we suggest that our self-organized spheroid model can be utilized as a new in vitro model to represent the osteocyte and to recapitulate an in vitro ossification process.
format	article
author	Jeonghyun Kim Taiji Adachi
author_facet	Jeonghyun Kim Taiji Adachi
author_sort	Jeonghyun Kim
title	Cell-fate decision of mesenchymal stem cells toward osteocyte differentiation is committed by spheroid culture
title_short	Cell-fate decision of mesenchymal stem cells toward osteocyte differentiation is committed by spheroid culture
title_full	Cell-fate decision of mesenchymal stem cells toward osteocyte differentiation is committed by spheroid culture
title_fullStr	Cell-fate decision of mesenchymal stem cells toward osteocyte differentiation is committed by spheroid culture
title_full_unstemmed	Cell-fate decision of mesenchymal stem cells toward osteocyte differentiation is committed by spheroid culture
title_sort	cell-fate decision of mesenchymal stem cells toward osteocyte differentiation is committed by spheroid culture
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2425ffd5bce84a2d9252cadcd93ffc76
work_keys_str_mv	AT jeonghyunkim cellfatedecisionofmesenchymalstemcellstowardosteocytedifferentiationiscommittedbyspheroidculture AT taijiadachi cellfatedecisionofmesenchymalstemcellstowardosteocytedifferentiationiscommittedbyspheroidculture
_version_	1718381366038495232

Cell-fate decision of mesenchymal stem cells toward osteocyte differentiation is committed by spheroid culture

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