Interspecific germline transmission of cultured primordial germ cells.

In birds, the primordial germ cell (PGC) lineage separates from the soma within 24 h following fertilization. Here we show that the endogenous population of about 200 PGCs from a single chicken embryo can be expanded one million fold in culture. When cultured PGCs are injected into a xenogeneic embr...

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Autores principales:	Marie-Cecile van de Lavoir, Ellen J Collarini, Philip A Leighton, Jeffrey Fesler, Daniel R Lu, William D Harriman, T S Thiyagasundaram, Robert J Etches
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/243d6e2898f243b7a3cec31eabb968bc
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spelling	oai:doaj.org-article:243d6e2898f243b7a3cec31eabb968bc2021-11-18T07:17:59ZInterspecific germline transmission of cultured primordial germ cells.1932-620310.1371/journal.pone.0035664https://doaj.org/article/243d6e2898f243b7a3cec31eabb968bc2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22629301/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203In birds, the primordial germ cell (PGC) lineage separates from the soma within 24 h following fertilization. Here we show that the endogenous population of about 200 PGCs from a single chicken embryo can be expanded one million fold in culture. When cultured PGCs are injected into a xenogeneic embryo at an equivalent stage of development, they colonize the testis. At sexual maturity, these donor PGCs undergo spermatogenesis in the xenogeneic host and become functional sperm. Insemination of semen from the xenogeneic host into females from the donor species produces normal offspring from the donor species. In our model system, the donor species is chicken (Gallus domesticus) and the recipient species is guinea fowl (Numida meleagris), a member of a different avian family, suggesting that the mechanisms controlling proliferation of the germline are highly conserved within birds. From a pragmatic perspective, these data are the basis of a novel strategy to produce endangered species of birds using domesticated hosts that are both tractable and fecund.Marie-Cecile van de LavoirEllen J CollariniPhilip A LeightonJeffrey FeslerDaniel R LuWilliam D HarrimanT S ThiyagasundaramRobert J EtchesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e35664 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marie-Cecile van de Lavoir Ellen J Collarini Philip A Leighton Jeffrey Fesler Daniel R Lu William D Harriman T S Thiyagasundaram Robert J Etches Interspecific germline transmission of cultured primordial germ cells.
description	In birds, the primordial germ cell (PGC) lineage separates from the soma within 24 h following fertilization. Here we show that the endogenous population of about 200 PGCs from a single chicken embryo can be expanded one million fold in culture. When cultured PGCs are injected into a xenogeneic embryo at an equivalent stage of development, they colonize the testis. At sexual maturity, these donor PGCs undergo spermatogenesis in the xenogeneic host and become functional sperm. Insemination of semen from the xenogeneic host into females from the donor species produces normal offspring from the donor species. In our model system, the donor species is chicken (Gallus domesticus) and the recipient species is guinea fowl (Numida meleagris), a member of a different avian family, suggesting that the mechanisms controlling proliferation of the germline are highly conserved within birds. From a pragmatic perspective, these data are the basis of a novel strategy to produce endangered species of birds using domesticated hosts that are both tractable and fecund.
format	article
author	Marie-Cecile van de Lavoir Ellen J Collarini Philip A Leighton Jeffrey Fesler Daniel R Lu William D Harriman T S Thiyagasundaram Robert J Etches
author_facet	Marie-Cecile van de Lavoir Ellen J Collarini Philip A Leighton Jeffrey Fesler Daniel R Lu William D Harriman T S Thiyagasundaram Robert J Etches
author_sort	Marie-Cecile van de Lavoir
title	Interspecific germline transmission of cultured primordial germ cells.
title_short	Interspecific germline transmission of cultured primordial germ cells.
title_full	Interspecific germline transmission of cultured primordial germ cells.
title_fullStr	Interspecific germline transmission of cultured primordial germ cells.
title_full_unstemmed	Interspecific germline transmission of cultured primordial germ cells.
title_sort	interspecific germline transmission of cultured primordial germ cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/243d6e2898f243b7a3cec31eabb968bc
work_keys_str_mv	AT mariececilevandelavoir interspecificgermlinetransmissionofculturedprimordialgermcells AT ellenjcollarini interspecificgermlinetransmissionofculturedprimordialgermcells AT philipaleighton interspecificgermlinetransmissionofculturedprimordialgermcells AT jeffreyfesler interspecificgermlinetransmissionofculturedprimordialgermcells AT danielrlu interspecificgermlinetransmissionofculturedprimordialgermcells AT williamdharriman interspecificgermlinetransmissionofculturedprimordialgermcells AT tsthiyagasundaram interspecificgermlinetransmissionofculturedprimordialgermcells AT robertjetches interspecificgermlinetransmissionofculturedprimordialgermcells
_version_	1718423650440314880

Interspecific germline transmission of cultured primordial germ cells.

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