Rapid, Selection-Free, High-Efficiency Genome Editing in Protozoan Parasites Using CRISPR-Cas9 Ribonucleoproteins

ABSTRACT Trypanosomatids (order Kinetoplastida), including the human pathogens Trypanosoma cruzi (agent of Chagas disease), Trypanosoma brucei, (African sleeping sickness), and Leishmania (leishmaniasis), affect millions of people and animals globally. T. cruzi is considered one of the least studied...

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Autores principales: Lia Carolina Soares Medeiros, Lilith South, Duo Peng, Juan M. Bustamante, Wei Wang, Molly Bunkofske, Natasha Perumal, Fernando Sanchez-Valdez, Rick L. Tarleton
Formato: article
Lenguaje:EN
Publicado: American Society for Microbiology 2017
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Acceso en línea:https://doaj.org/article/24601f0528a048dc8f67bc65964beaaa
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