In vitro RNase and nucleic acid binding activities implicate coilin in U snRNA processing.

Coilin is known as the marker protein for Cajal bodies (CBs), subnuclear domains important for the biogenesis of small nuclear ribonucleoproteins (snRNPs) which function in pre-mRNA splicing. CBs associate non-randomly with U1 and U2 gene loci, which produce the small nuclear RNA (snRNA) component o...

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Autores principales:	Hanna J Broome, Michael D Hebert
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/24bd3892fa954e779668aadc02bce967
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spelling	oai:doaj.org-article:24bd3892fa954e779668aadc02bce9672021-11-18T07:20:17ZIn vitro RNase and nucleic acid binding activities implicate coilin in U snRNA processing.1932-620310.1371/journal.pone.0036300https://doaj.org/article/24bd3892fa954e779668aadc02bce9672012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22558428/?tool=EBIhttps://doaj.org/toc/1932-6203Coilin is known as the marker protein for Cajal bodies (CBs), subnuclear domains important for the biogenesis of small nuclear ribonucleoproteins (snRNPs) which function in pre-mRNA splicing. CBs associate non-randomly with U1 and U2 gene loci, which produce the small nuclear RNA (snRNA) component of the respective snRNP. Despite recognition as the CB marker protein, coilin is primarily nucleoplasmic, and the function of this fraction is not fully characterized. Here we show that coilin binds double stranded DNA and has RNase activity in vitro. U1 and U2 snRNAs undergo a processing event of the primary transcript prior to incorporation in the snRNP. We find that coilin displays RNase activity within the CU region of the U2 snRNA primary transcript in vitro, and that coilin knockdown results in accumulation of the 3' pre-processed U1 and U2 snRNA. These findings present new characteristics of coilin in vitro, and suggest additional functions of the protein in vivo.Hanna J BroomeMichael D HebertPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e36300 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hanna J Broome Michael D Hebert In vitro RNase and nucleic acid binding activities implicate coilin in U snRNA processing.
description	Coilin is known as the marker protein for Cajal bodies (CBs), subnuclear domains important for the biogenesis of small nuclear ribonucleoproteins (snRNPs) which function in pre-mRNA splicing. CBs associate non-randomly with U1 and U2 gene loci, which produce the small nuclear RNA (snRNA) component of the respective snRNP. Despite recognition as the CB marker protein, coilin is primarily nucleoplasmic, and the function of this fraction is not fully characterized. Here we show that coilin binds double stranded DNA and has RNase activity in vitro. U1 and U2 snRNAs undergo a processing event of the primary transcript prior to incorporation in the snRNP. We find that coilin displays RNase activity within the CU region of the U2 snRNA primary transcript in vitro, and that coilin knockdown results in accumulation of the 3' pre-processed U1 and U2 snRNA. These findings present new characteristics of coilin in vitro, and suggest additional functions of the protein in vivo.
format	article
author	Hanna J Broome Michael D Hebert
author_facet	Hanna J Broome Michael D Hebert
author_sort	Hanna J Broome
title	In vitro RNase and nucleic acid binding activities implicate coilin in U snRNA processing.
title_short	In vitro RNase and nucleic acid binding activities implicate coilin in U snRNA processing.
title_full	In vitro RNase and nucleic acid binding activities implicate coilin in U snRNA processing.
title_fullStr	In vitro RNase and nucleic acid binding activities implicate coilin in U snRNA processing.
title_full_unstemmed	In vitro RNase and nucleic acid binding activities implicate coilin in U snRNA processing.
title_sort	in vitro rnase and nucleic acid binding activities implicate coilin in u snrna processing.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/24bd3892fa954e779668aadc02bce967
work_keys_str_mv	AT hannajbroome invitrornaseandnucleicacidbindingactivitiesimplicatecoilininusnrnaprocessing AT michaeldhebert invitrornaseandnucleicacidbindingactivitiesimplicatecoilininusnrnaprocessing
_version_	1718423584442941440

In vitro RNase and nucleic acid binding activities implicate coilin in U snRNA processing.

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