Molecular characterization of lactic acid bacteria producing edible biofilm isolated from kimchi

Biofilm is a community of microorganisms that interrelated and covered by an extracellular polymer matrix. This biofilm can be produced by Lactic acid bacteria (LAB) which was edible for human and animal. Therefore, it possible to be applied in the food and health industry. One source of LAB is kim...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	FADILLA SAPALINA, Endah Retnaningrum
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	extracellular polymer matrix, lactobacillus brevis, 16s rrna gene, zeolite Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/25ac6e0a59bb47bd8403a29d7612173c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:25ac6e0a59bb47bd8403a29d7612173c
record_format	dspace
spelling	oai:doaj.org-article:25ac6e0a59bb47bd8403a29d7612173c2021-11-22T00:28:56ZMolecular characterization of lactic acid bacteria producing edible biofilm isolated from kimchi1412-033X2085-472210.13057/biodiv/d210315https://doaj.org/article/25ac6e0a59bb47bd8403a29d7612173c2020-02-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4961https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Biofilm is a community of microorganisms that interrelated and covered by an extracellular polymer matrix. This biofilm can be produced by Lactic acid bacteria (LAB) which was edible for human and animal. Therefore, it possible to be applied in the food and health industry. One source of LAB is kimchi, as fermented food from Korea and it has good benefits for health. This research aimed to obtain LAB from kimchi that can produce edible biofilms and to identify LAB producing edible biofilms based on the 16S rRNA gene. Isolation of lactic acid bacteria from kimchi cultured in MRS agar medium containing 1% CaCO3, biofilm production test with biofilm assay method and zeolite as a substrate to adherent cells. For amplification of 16S rRNA gene used primer 27F and 1492R. The isolate of KA2, KA5, KB1, and KC4 isolated from kimchi and could produce biofilms with the highest biofilm formation at 48 hours incubation time. Based on molecular identification with 16S rRNA gene sequencing, the four isolates identified as Lactobacillus brevis species.FADILLA SAPALINAEndah RetnaningrumMBI & UNS Soloarticleextracellular polymer matrix, lactobacillus brevis, 16s rrna gene, zeoliteBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 3 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	extracellular polymer matrix, lactobacillus brevis, 16s rrna gene, zeolite Biology (General) QH301-705.5
spellingShingle	extracellular polymer matrix, lactobacillus brevis, 16s rrna gene, zeolite Biology (General) QH301-705.5 FADILLA SAPALINA Endah Retnaningrum Molecular characterization of lactic acid bacteria producing edible biofilm isolated from kimchi
description	Biofilm is a community of microorganisms that interrelated and covered by an extracellular polymer matrix. This biofilm can be produced by Lactic acid bacteria (LAB) which was edible for human and animal. Therefore, it possible to be applied in the food and health industry. One source of LAB is kimchi, as fermented food from Korea and it has good benefits for health. This research aimed to obtain LAB from kimchi that can produce edible biofilms and to identify LAB producing edible biofilms based on the 16S rRNA gene. Isolation of lactic acid bacteria from kimchi cultured in MRS agar medium containing 1% CaCO3, biofilm production test with biofilm assay method and zeolite as a substrate to adherent cells. For amplification of 16S rRNA gene used primer 27F and 1492R. The isolate of KA2, KA5, KB1, and KC4 isolated from kimchi and could produce biofilms with the highest biofilm formation at 48 hours incubation time. Based on molecular identification with 16S rRNA gene sequencing, the four isolates identified as Lactobacillus brevis species.
format	article
author	FADILLA SAPALINA Endah Retnaningrum
author_facet	FADILLA SAPALINA Endah Retnaningrum
author_sort	FADILLA SAPALINA
title	Molecular characterization of lactic acid bacteria producing edible biofilm isolated from kimchi
title_short	Molecular characterization of lactic acid bacteria producing edible biofilm isolated from kimchi
title_full	Molecular characterization of lactic acid bacteria producing edible biofilm isolated from kimchi
title_fullStr	Molecular characterization of lactic acid bacteria producing edible biofilm isolated from kimchi
title_full_unstemmed	Molecular characterization of lactic acid bacteria producing edible biofilm isolated from kimchi
title_sort	molecular characterization of lactic acid bacteria producing edible biofilm isolated from kimchi
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/25ac6e0a59bb47bd8403a29d7612173c
work_keys_str_mv	AT fadillasapalina molecularcharacterizationoflacticacidbacteriaproducingediblebiofilmisolatedfromkimchi AT endahretnaningrum molecularcharacterizationoflacticacidbacteriaproducingediblebiofilmisolatedfromkimchi
_version_	1718418571577524224

Molecular characterization of lactic acid bacteria producing edible biofilm isolated from kimchi

Ejemplares similares