New environment-sensitive multichannel DNA fluorescent label for investigation of the protein-DNA interactions.
Here, we report the study of a new multichannel DNA fluorescent base analogue 3-hydroxychromone (3HC) to evaluate its suitability as a fluorescent reporter probe of structural transitions during protein-DNA interactions and its comparison with the current commercially available 2-aminopurine (aPu),...
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2014
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oai:doaj.org-article:25e756899cb94e80bdac07a9dbccbea32021-11-18T08:15:52ZNew environment-sensitive multichannel DNA fluorescent label for investigation of the protein-DNA interactions.1932-620310.1371/journal.pone.0100007https://doaj.org/article/25e756899cb94e80bdac07a9dbccbea32014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24925085/?tool=EBIhttps://doaj.org/toc/1932-6203Here, we report the study of a new multichannel DNA fluorescent base analogue 3-hydroxychromone (3HC) to evaluate its suitability as a fluorescent reporter probe of structural transitions during protein-DNA interactions and its comparison with the current commercially available 2-aminopurine (aPu), pyrrolocytosine (Cpy) and 1,3-diaza-2-oxophenoxazine (tCO). For this purpose, fluorescent base analogues were incorporated into DNA helix on the opposite or on the 5'-side of the damaged nucleoside 5,6-dihydrouridine (DHU), which is specifically recognized and removed by Endonuclease VIII. These fluorophores demonstrated different sensitivities to the DNA helix conformational changes. The highest sensitivity and the most detailed information about the conformational changes of DNA induced by protein binding and processing were obtained using the 3HC probe. The application of this new artificial fluorescent DNA base is a very useful tool for the studies of complex mechanisms of protein-DNA interactions. Using 3HC biosensor, the kinetic mechanism of Endonuclease VIII action was specified.Alexandra A KuznetsovaNikita A KuznetsovYuri N VorobjevNicolas P F BarthesBenoît Y MichelAlain BurgerOlga S FedorovaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e100007 (2014) |
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Medicine R Science Q Alexandra A Kuznetsova Nikita A Kuznetsov Yuri N Vorobjev Nicolas P F Barthes Benoît Y Michel Alain Burger Olga S Fedorova New environment-sensitive multichannel DNA fluorescent label for investigation of the protein-DNA interactions. |
| description |
Here, we report the study of a new multichannel DNA fluorescent base analogue 3-hydroxychromone (3HC) to evaluate its suitability as a fluorescent reporter probe of structural transitions during protein-DNA interactions and its comparison with the current commercially available 2-aminopurine (aPu), pyrrolocytosine (Cpy) and 1,3-diaza-2-oxophenoxazine (tCO). For this purpose, fluorescent base analogues were incorporated into DNA helix on the opposite or on the 5'-side of the damaged nucleoside 5,6-dihydrouridine (DHU), which is specifically recognized and removed by Endonuclease VIII. These fluorophores demonstrated different sensitivities to the DNA helix conformational changes. The highest sensitivity and the most detailed information about the conformational changes of DNA induced by protein binding and processing were obtained using the 3HC probe. The application of this new artificial fluorescent DNA base is a very useful tool for the studies of complex mechanisms of protein-DNA interactions. Using 3HC biosensor, the kinetic mechanism of Endonuclease VIII action was specified. |
| format |
article |
| author |
Alexandra A Kuznetsova Nikita A Kuznetsov Yuri N Vorobjev Nicolas P F Barthes Benoît Y Michel Alain Burger Olga S Fedorova |
| author_facet |
Alexandra A Kuznetsova Nikita A Kuznetsov Yuri N Vorobjev Nicolas P F Barthes Benoît Y Michel Alain Burger Olga S Fedorova |
| author_sort |
Alexandra A Kuznetsova |
| title |
New environment-sensitive multichannel DNA fluorescent label for investigation of the protein-DNA interactions. |
| title_short |
New environment-sensitive multichannel DNA fluorescent label for investigation of the protein-DNA interactions. |
| title_full |
New environment-sensitive multichannel DNA fluorescent label for investigation of the protein-DNA interactions. |
| title_fullStr |
New environment-sensitive multichannel DNA fluorescent label for investigation of the protein-DNA interactions. |
| title_full_unstemmed |
New environment-sensitive multichannel DNA fluorescent label for investigation of the protein-DNA interactions. |
| title_sort |
new environment-sensitive multichannel dna fluorescent label for investigation of the protein-dna interactions. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/25e756899cb94e80bdac07a9dbccbea3 |
| work_keys_str_mv |
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1718421972750172160 |