Validation of mathematical model with phosphate activation effect by batch (R)-phenylacetylcarbinol biotransformation process utilizing Candida tropicalis pyruvate decarboxylase in phosphate buffer

Validation of mathematical model with phosphate activation effect by batch (R)-phenylacetylcarbinol biotransformation process utilizing Candida tropicalis pyruvate decarboxylase in phosphate buffer

Abstract The (R)-phenylacetylcarbinol (PAC) batch biotransformation kinetics for partially purified Candida tropicalis TISTR 5350 pyruvate decarboxylase (PDC) were determined to validate a comprehensive mathematical model in 250 mL scale with 250 mM phosphate buffer/pH 7.0. PDC could convert initial...

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Main Authors: Julaluk Khemacheewakul, Siraphat Taesuwan, Rojarej Nunta, Charin Techapun, Yuthana Phimolsiripol, Pornchai Rachtanapun, Kittisak Jantanasakulwong, Kritsadaporn Porninta, Sumeth Sommanee, Chatchadaporn Mahakuntha, Thanongsak Chaiyaso, Phisit Seesuriyachan, Alissara Reungsang, Ngoc Thao Ngan Trinh, Sutee Wangtueai, Sarana Rose Sommano, Noppol Leksawasdi
Format: article
Language:EN
Published: Nature Portfolio 2021
Subjects:
Medicine
R
Science
Q
Online Access:https://doaj.org/article/25e81d906cd44955a53fa81fbb4ae9f4
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