Massive QTL analysis identifies pleiotropic genetic determinants for stress resistance, aroma formation, and ethanol, glycerol and isobutanol production in Saccharomyces cerevisiae

Abstract Background The brewer’s yeast Saccharomyces cerevisiae is exploited in several industrial processes, ranging from food and beverage fermentation to the production of biofuels, pharmaceuticals and complex chemicals. The large genetic and phenotypic diversity within this species offers a form...

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Autores principales:	Ping-Wei Ho, Supinya Piampongsant, Brigida Gallone, Andrea Del Cortona, Pieter-Jan Peeters, Frank Reijbroek, Jules Verbaet, Beatriz Herrera, Jeroen Cortebeeck, Robbe Nolmans, Veerle Saels, Jan Steensels, Daniel F. Jarosz, Kevin J. Verstrepen
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Fuel TP315-360 Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/26a80d9fab4e47b38ee03e7ef549f1a3
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spelling	oai:doaj.org-article:26a80d9fab4e47b38ee03e7ef549f1a32021-11-08T10:56:40ZMassive QTL analysis identifies pleiotropic genetic determinants for stress resistance, aroma formation, and ethanol, glycerol and isobutanol production in Saccharomyces cerevisiae10.1186/s13068-021-02059-w1754-6834https://doaj.org/article/26a80d9fab4e47b38ee03e7ef549f1a32021-11-01T00:00:00Zhttps://doi.org/10.1186/s13068-021-02059-whttps://doaj.org/toc/1754-6834Abstract Background The brewer’s yeast Saccharomyces cerevisiae is exploited in several industrial processes, ranging from food and beverage fermentation to the production of biofuels, pharmaceuticals and complex chemicals. The large genetic and phenotypic diversity within this species offers a formidable natural resource to obtain superior strains, hybrids, and variants. However, most industrially relevant traits in S. cerevisiae strains are controlled by multiple genetic loci. Over the past years, several studies have identified some of these QTLs. However, because these studies only focus on a limited set of traits and often use different techniques and starting strains, a global view of industrially relevant QTLs is still missing. Results Here, we combined the power of 1125 fully sequenced inbred segregants with high-throughput phenotyping methods to identify as many as 678 QTLs across 18 different traits relevant to industrial fermentation processes, including production of ethanol, glycerol, isobutanol, acetic acid, sulfur dioxide, flavor-active esters, as well as resistance to ethanol, acetic acid, sulfite and high osmolarity. We identified and confirmed several variants that are associated with multiple different traits, indicating that many QTLs are pleiotropic. Moreover, we show that both rare and common variants, as well as variants located in coding and non-coding regions all contribute to the phenotypic variation. Conclusions Our findings represent an important step in our understanding of the genetic underpinnings of industrially relevant yeast traits and open new routes to study complex genetics and genetic interactions as well as to engineer novel, superior industrial yeasts. Moreover, the major role of rare variants suggests that there is a plethora of different combinations of mutations that can be explored in genome editing.Ping-Wei HoSupinya PiampongsantBrigida GalloneAndrea Del CortonaPieter-Jan PeetersFrank ReijbroekJules VerbaetBeatriz HerreraJeroen CortebeeckRobbe NolmansVeerle SaelsJan SteenselsDaniel F. JaroszKevin J. VerstrepenBMCarticleFuelTP315-360BiotechnologyTP248.13-248.65ENBiotechnology for Biofuels, Vol 14, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Fuel TP315-360 Biotechnology TP248.13-248.65
spellingShingle	Fuel TP315-360 Biotechnology TP248.13-248.65 Ping-Wei Ho Supinya Piampongsant Brigida Gallone Andrea Del Cortona Pieter-Jan Peeters Frank Reijbroek Jules Verbaet Beatriz Herrera Jeroen Cortebeeck Robbe Nolmans Veerle Saels Jan Steensels Daniel F. Jarosz Kevin J. Verstrepen Massive QTL analysis identifies pleiotropic genetic determinants for stress resistance, aroma formation, and ethanol, glycerol and isobutanol production in Saccharomyces cerevisiae
description	Abstract Background The brewer’s yeast Saccharomyces cerevisiae is exploited in several industrial processes, ranging from food and beverage fermentation to the production of biofuels, pharmaceuticals and complex chemicals. The large genetic and phenotypic diversity within this species offers a formidable natural resource to obtain superior strains, hybrids, and variants. However, most industrially relevant traits in S. cerevisiae strains are controlled by multiple genetic loci. Over the past years, several studies have identified some of these QTLs. However, because these studies only focus on a limited set of traits and often use different techniques and starting strains, a global view of industrially relevant QTLs is still missing. Results Here, we combined the power of 1125 fully sequenced inbred segregants with high-throughput phenotyping methods to identify as many as 678 QTLs across 18 different traits relevant to industrial fermentation processes, including production of ethanol, glycerol, isobutanol, acetic acid, sulfur dioxide, flavor-active esters, as well as resistance to ethanol, acetic acid, sulfite and high osmolarity. We identified and confirmed several variants that are associated with multiple different traits, indicating that many QTLs are pleiotropic. Moreover, we show that both rare and common variants, as well as variants located in coding and non-coding regions all contribute to the phenotypic variation. Conclusions Our findings represent an important step in our understanding of the genetic underpinnings of industrially relevant yeast traits and open new routes to study complex genetics and genetic interactions as well as to engineer novel, superior industrial yeasts. Moreover, the major role of rare variants suggests that there is a plethora of different combinations of mutations that can be explored in genome editing.
format	article
author	Ping-Wei Ho Supinya Piampongsant Brigida Gallone Andrea Del Cortona Pieter-Jan Peeters Frank Reijbroek Jules Verbaet Beatriz Herrera Jeroen Cortebeeck Robbe Nolmans Veerle Saels Jan Steensels Daniel F. Jarosz Kevin J. Verstrepen
author_facet	Ping-Wei Ho Supinya Piampongsant Brigida Gallone Andrea Del Cortona Pieter-Jan Peeters Frank Reijbroek Jules Verbaet Beatriz Herrera Jeroen Cortebeeck Robbe Nolmans Veerle Saels Jan Steensels Daniel F. Jarosz Kevin J. Verstrepen
author_sort	Ping-Wei Ho
title	Massive QTL analysis identifies pleiotropic genetic determinants for stress resistance, aroma formation, and ethanol, glycerol and isobutanol production in Saccharomyces cerevisiae
title_short	Massive QTL analysis identifies pleiotropic genetic determinants for stress resistance, aroma formation, and ethanol, glycerol and isobutanol production in Saccharomyces cerevisiae
title_full	Massive QTL analysis identifies pleiotropic genetic determinants for stress resistance, aroma formation, and ethanol, glycerol and isobutanol production in Saccharomyces cerevisiae
title_fullStr	Massive QTL analysis identifies pleiotropic genetic determinants for stress resistance, aroma formation, and ethanol, glycerol and isobutanol production in Saccharomyces cerevisiae
title_full_unstemmed	Massive QTL analysis identifies pleiotropic genetic determinants for stress resistance, aroma formation, and ethanol, glycerol and isobutanol production in Saccharomyces cerevisiae
title_sort	massive qtl analysis identifies pleiotropic genetic determinants for stress resistance, aroma formation, and ethanol, glycerol and isobutanol production in saccharomyces cerevisiae
publisher	BMC
publishDate	2021
url	https://doaj.org/article/26a80d9fab4e47b38ee03e7ef549f1a3
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Massive QTL analysis identifies pleiotropic genetic determinants for stress resistance, aroma formation, and ethanol, glycerol and isobutanol production in Saccharomyces cerevisiae

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