Histone deacetylase 6 (HDAC6) is an essential factor for oocyte maturation and asymmetric division in mice

Abstract Tubastatin A (Tub-A), a highly selective histone deacetylase 6 (HDAC6) inhibitor, has been widely used as a cytotoxic anticancer agent, or for the treatment of patients with asthma. However, the potential toxicity of Tub-A on oocyte maturation and asymmetric division is still unclear. There...

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Autores principales:	Dongjie Zhou, Yun-Jung Choi, Jin-Hoi Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/26af42e37017422b90f02f3e7c81edb5
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spelling	oai:doaj.org-article:26af42e37017422b90f02f3e7c81edb52021-12-02T12:32:56ZHistone deacetylase 6 (HDAC6) is an essential factor for oocyte maturation and asymmetric division in mice10.1038/s41598-017-08650-22045-2322https://doaj.org/article/26af42e37017422b90f02f3e7c81edb52017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08650-2https://doaj.org/toc/2045-2322Abstract Tubastatin A (Tub-A), a highly selective histone deacetylase 6 (HDAC6) inhibitor, has been widely used as a cytotoxic anticancer agent, or for the treatment of patients with asthma. However, the potential toxicity of Tub-A on oocyte maturation and asymmetric division is still unclear. Therefore, the present study was designed to examine the effect and potential regulatory role of Tub-A on the meiotic maturation of oocytes. We observed that Tub-A treatment induced an increased level of the acetylation of α-tubulin, and a failure of spindle migration and actin cap formation. Based on the spindle structure, most Tub-A treated oocytes were arrested in an MI-like or a GVBD-like stage and exhibited decondensed chromosomes in a dose dependent manner. Moreover, Tub-A treatment decreased the protein expression of mTOR, a factor responsible for spindle formation, and the expression of mDia1, an inhibitor of actin assembly, in an HDAC6 expression-dependent manner. Importantly, following Tub-A supplementation, most oocytes failed to extrude the first polar body, which indicates that these defects are closely linked to abnormal oocyte maturation. Taken together, our data demonstrates that HDAC6 is one of the essential factors for oocyte maturation and asymmetric division via the HDAC6/mTOR or mDia1 pathway in mice.Dongjie ZhouYun-Jung ChoiJin-Hoi KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dongjie Zhou Yun-Jung Choi Jin-Hoi Kim Histone deacetylase 6 (HDAC6) is an essential factor for oocyte maturation and asymmetric division in mice
description	Abstract Tubastatin A (Tub-A), a highly selective histone deacetylase 6 (HDAC6) inhibitor, has been widely used as a cytotoxic anticancer agent, or for the treatment of patients with asthma. However, the potential toxicity of Tub-A on oocyte maturation and asymmetric division is still unclear. Therefore, the present study was designed to examine the effect and potential regulatory role of Tub-A on the meiotic maturation of oocytes. We observed that Tub-A treatment induced an increased level of the acetylation of α-tubulin, and a failure of spindle migration and actin cap formation. Based on the spindle structure, most Tub-A treated oocytes were arrested in an MI-like or a GVBD-like stage and exhibited decondensed chromosomes in a dose dependent manner. Moreover, Tub-A treatment decreased the protein expression of mTOR, a factor responsible for spindle formation, and the expression of mDia1, an inhibitor of actin assembly, in an HDAC6 expression-dependent manner. Importantly, following Tub-A supplementation, most oocytes failed to extrude the first polar body, which indicates that these defects are closely linked to abnormal oocyte maturation. Taken together, our data demonstrates that HDAC6 is one of the essential factors for oocyte maturation and asymmetric division via the HDAC6/mTOR or mDia1 pathway in mice.
format	article
author	Dongjie Zhou Yun-Jung Choi Jin-Hoi Kim
author_facet	Dongjie Zhou Yun-Jung Choi Jin-Hoi Kim
author_sort	Dongjie Zhou
title	Histone deacetylase 6 (HDAC6) is an essential factor for oocyte maturation and asymmetric division in mice
title_short	Histone deacetylase 6 (HDAC6) is an essential factor for oocyte maturation and asymmetric division in mice
title_full	Histone deacetylase 6 (HDAC6) is an essential factor for oocyte maturation and asymmetric division in mice
title_fullStr	Histone deacetylase 6 (HDAC6) is an essential factor for oocyte maturation and asymmetric division in mice
title_full_unstemmed	Histone deacetylase 6 (HDAC6) is an essential factor for oocyte maturation and asymmetric division in mice
title_sort	histone deacetylase 6 (hdac6) is an essential factor for oocyte maturation and asymmetric division in mice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/26af42e37017422b90f02f3e7c81edb5
work_keys_str_mv	AT dongjiezhou histonedeacetylase6hdac6isanessentialfactorforoocytematurationandasymmetricdivisioninmice AT yunjungchoi histonedeacetylase6hdac6isanessentialfactorforoocytematurationandasymmetricdivisioninmice AT jinhoikim histonedeacetylase6hdac6isanessentialfactorforoocytematurationandasymmetricdivisioninmice
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Histone deacetylase 6 (HDAC6) is an essential factor for oocyte maturation and asymmetric division in mice

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