Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale
Combining improved sample preparation, data-independent acquisition mass spectrometry and deep learning, the authors develop a workflow for more robust and precise quantitative ubiquitinome profiling. They use this method to characterize targets of the deubiquitinase USP7 and effects of USP7 inhibit...
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Main Authors: | Martin Steger, Vadim Demichev, Mattias Backman, Uli Ohmayer, Phillip Ihmor, Stefan Müller, Markus Ralser, Henrik Daub |
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Format: | article |
Language: | EN |
Published: |
Nature Portfolio
2021
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Online Access: | https://doaj.org/article/284693f5e9d14df9b7b8bbbd91efb49e |
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