Proteomics-based screening of the target proteins associated with antidepressant-like effect and mechanism of nimesulide

Abstract Nimesulide is an inhibitor of COX-2 with antioxidant and anti-inflammatory effects. However, few studies have explored the antidepressant mechanism of nimesulide. Here, we evaluated the therapeutic effects of nimesulide on CUMS rats. iTRAQ technology was used to identify the differentially...

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Autores principales:	Wen Luo, Ying Luo, Junqing Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2904b7fe732740e892b41441350fcaaf
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spelling	oai:doaj.org-article:2904b7fe732740e892b41441350fcaaf2021-12-02T15:39:49ZProteomics-based screening of the target proteins associated with antidepressant-like effect and mechanism of nimesulide10.1038/s41598-020-66420-z2045-2322https://doaj.org/article/2904b7fe732740e892b41441350fcaaf2020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-66420-zhttps://doaj.org/toc/2045-2322Abstract Nimesulide is an inhibitor of COX-2 with antioxidant and anti-inflammatory effects. However, few studies have explored the antidepressant mechanism of nimesulide. Here, we evaluated the therapeutic effects of nimesulide on CUMS rats. iTRAQ technology was used to identify the differentially expressed protein in the hippocampus between CUMS and nimesulide-treated rats to identify the possible molecular mechanism of its effects. We found that nimesulide had positive effects on depressive-like behaviors and inflammatory factors in depressed rats. Using proteomics technologies, we screened 16 differentially expressed proteins in CUMS-exposed rats after nimesulide treatment, 5 of which were related to inflammation. Overall, these results show that nimesulide might mediate its antidepressant effect on depressed rats through the inhibition of oxidative stress inflammatory response.Wen LuoYing LuoJunqing YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wen Luo Ying Luo Junqing Yang Proteomics-based screening of the target proteins associated with antidepressant-like effect and mechanism of nimesulide
description	Abstract Nimesulide is an inhibitor of COX-2 with antioxidant and anti-inflammatory effects. However, few studies have explored the antidepressant mechanism of nimesulide. Here, we evaluated the therapeutic effects of nimesulide on CUMS rats. iTRAQ technology was used to identify the differentially expressed protein in the hippocampus between CUMS and nimesulide-treated rats to identify the possible molecular mechanism of its effects. We found that nimesulide had positive effects on depressive-like behaviors and inflammatory factors in depressed rats. Using proteomics technologies, we screened 16 differentially expressed proteins in CUMS-exposed rats after nimesulide treatment, 5 of which were related to inflammation. Overall, these results show that nimesulide might mediate its antidepressant effect on depressed rats through the inhibition of oxidative stress inflammatory response.
format	article
author	Wen Luo Ying Luo Junqing Yang
author_facet	Wen Luo Ying Luo Junqing Yang
author_sort	Wen Luo
title	Proteomics-based screening of the target proteins associated with antidepressant-like effect and mechanism of nimesulide
title_short	Proteomics-based screening of the target proteins associated with antidepressant-like effect and mechanism of nimesulide
title_full	Proteomics-based screening of the target proteins associated with antidepressant-like effect and mechanism of nimesulide
title_fullStr	Proteomics-based screening of the target proteins associated with antidepressant-like effect and mechanism of nimesulide
title_full_unstemmed	Proteomics-based screening of the target proteins associated with antidepressant-like effect and mechanism of nimesulide
title_sort	proteomics-based screening of the target proteins associated with antidepressant-like effect and mechanism of nimesulide
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/2904b7fe732740e892b41441350fcaaf
work_keys_str_mv	AT wenluo proteomicsbasedscreeningofthetargetproteinsassociatedwithantidepressantlikeeffectandmechanismofnimesulide AT yingluo proteomicsbasedscreeningofthetargetproteinsassociatedwithantidepressantlikeeffectandmechanismofnimesulide AT junqingyang proteomicsbasedscreeningofthetargetproteinsassociatedwithantidepressantlikeeffectandmechanismofnimesulide
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Proteomics-based screening of the target proteins associated with antidepressant-like effect and mechanism of nimesulide

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