UHPLC-MS/MS determination of varietal thiol precursors in Sauvignon Blanc grapes

Abstract Varietal thiol precursors in grapes are subject to metabolic changes during post-harvest treatments. Metabolic activity should therefore be limited after sampling to understand their biosynthesis in the berry and genetic regulation. In this study, berries were frozen in liquid nitrogen imme...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Andreja Vanzo, Lucija Janeš, Franc Požgan, Špela Velikonja Bolta, Paolo Sivilotti, Klemen Lisjak
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/292e83d2cf0449f7ad9b377fb471d89c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:292e83d2cf0449f7ad9b377fb471d89c
record_format	dspace
spelling	oai:doaj.org-article:292e83d2cf0449f7ad9b377fb471d89c2021-12-02T15:05:23ZUHPLC-MS/MS determination of varietal thiol precursors in Sauvignon Blanc grapes10.1038/s41598-017-13273-82045-2322https://doaj.org/article/292e83d2cf0449f7ad9b377fb471d89c2017-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-13273-8https://doaj.org/toc/2045-2322Abstract Varietal thiol precursors in grapes are subject to metabolic changes during post-harvest treatments. Metabolic activity should therefore be limited after sampling to understand their biosynthesis in the berry and genetic regulation. In this study, berries were frozen in liquid nitrogen immediately after harvesting, transported in dry ice, stored briefly at −80 °C, cryo-milled and extracted without being thawed in cold methanol in a ratio of 1:4 (w/v). A UHPLC-MS/MS method for quantitative determination of the thiol precursors 3-S-glutathionylhexan-1-ol (G3MH), 3-S-cysteinylhexan-1-ol (Cys3MH), 4-S-glutathionyl-4-methylpentan-2-one (G4MMP) and 4-S-cysteinyl-4-methylpentan-2-one (Cys4MMP), glutathione, oxidized glutathione and L-methionine in grapes was developed. Reference material was provided through synthesis of precursors and their deuterium labelled analogues. The average thiol precursor content in grapes in 2013–15 was in the range 8–16 μg kg−1 for G3MH, 1–6 μg kg−1 for Cys3MH, 1–4 μg kg−1 for Cys4MMP and 0.3 μg kg−1 for G4MMP. In 2013 and 2014, the highest precursor content in mature Sauvignon Blanc grapes from vineyards located in Italy regarded G3MH, followed by Cys3MH, Cys4MMP and G4MMP. In 2015, G3MH was again the most abundant precursor, but followed by Cys4MMP, Cys3MH and G4MMP.Andreja VanzoLucija JanešFranc PožganŠpela Velikonja BoltaPaolo SivilottiKlemen LisjakNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andreja Vanzo Lucija Janeš Franc Požgan Špela Velikonja Bolta Paolo Sivilotti Klemen Lisjak UHPLC-MS/MS determination of varietal thiol precursors in Sauvignon Blanc grapes
description	Abstract Varietal thiol precursors in grapes are subject to metabolic changes during post-harvest treatments. Metabolic activity should therefore be limited after sampling to understand their biosynthesis in the berry and genetic regulation. In this study, berries were frozen in liquid nitrogen immediately after harvesting, transported in dry ice, stored briefly at −80 °C, cryo-milled and extracted without being thawed in cold methanol in a ratio of 1:4 (w/v). A UHPLC-MS/MS method for quantitative determination of the thiol precursors 3-S-glutathionylhexan-1-ol (G3MH), 3-S-cysteinylhexan-1-ol (Cys3MH), 4-S-glutathionyl-4-methylpentan-2-one (G4MMP) and 4-S-cysteinyl-4-methylpentan-2-one (Cys4MMP), glutathione, oxidized glutathione and L-methionine in grapes was developed. Reference material was provided through synthesis of precursors and their deuterium labelled analogues. The average thiol precursor content in grapes in 2013–15 was in the range 8–16 μg kg−1 for G3MH, 1–6 μg kg−1 for Cys3MH, 1–4 μg kg−1 for Cys4MMP and 0.3 μg kg−1 for G4MMP. In 2013 and 2014, the highest precursor content in mature Sauvignon Blanc grapes from vineyards located in Italy regarded G3MH, followed by Cys3MH, Cys4MMP and G4MMP. In 2015, G3MH was again the most abundant precursor, but followed by Cys4MMP, Cys3MH and G4MMP.
format	article
author	Andreja Vanzo Lucija Janeš Franc Požgan Špela Velikonja Bolta Paolo Sivilotti Klemen Lisjak
author_facet	Andreja Vanzo Lucija Janeš Franc Požgan Špela Velikonja Bolta Paolo Sivilotti Klemen Lisjak
author_sort	Andreja Vanzo
title	UHPLC-MS/MS determination of varietal thiol precursors in Sauvignon Blanc grapes
title_short	UHPLC-MS/MS determination of varietal thiol precursors in Sauvignon Blanc grapes
title_full	UHPLC-MS/MS determination of varietal thiol precursors in Sauvignon Blanc grapes
title_fullStr	UHPLC-MS/MS determination of varietal thiol precursors in Sauvignon Blanc grapes
title_full_unstemmed	UHPLC-MS/MS determination of varietal thiol precursors in Sauvignon Blanc grapes
title_sort	uhplc-ms/ms determination of varietal thiol precursors in sauvignon blanc grapes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/292e83d2cf0449f7ad9b377fb471d89c
work_keys_str_mv	AT andrejavanzo uhplcmsmsdeterminationofvarietalthiolprecursorsinsauvignonblancgrapes AT lucijajanes uhplcmsmsdeterminationofvarietalthiolprecursorsinsauvignonblancgrapes AT francpozgan uhplcmsmsdeterminationofvarietalthiolprecursorsinsauvignonblancgrapes AT spelavelikonjabolta uhplcmsmsdeterminationofvarietalthiolprecursorsinsauvignonblancgrapes AT paolosivilotti uhplcmsmsdeterminationofvarietalthiolprecursorsinsauvignonblancgrapes AT klemenlisjak uhplcmsmsdeterminationofvarietalthiolprecursorsinsauvignonblancgrapes
_version_	1718388818657148928

UHPLC-MS/MS determination of varietal thiol precursors in Sauvignon Blanc grapes

Ejemplares similares